A molecular marker closely linked to the pepper cytoplasmic male sterility restorer gene and its obtaining method and application

A technology for restoring genes and molecular markers, which is applied in the fields of pepper genetics, breeding and molecular biology, can solve the problems of pepper breeding lacking cytoplasmic male sterility restoration gene markers, etc., so as to reduce the field planting scale and later identification workload, save money Manpower, the effect of speeding up the breeding process

Active Publication Date: 2021-03-02
HUAZHONG AGRI UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is still a lack of reliable cytoplasmic male sterility restoration gene markers in pepper breeding. Based on this, this application is made

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A molecular marker closely linked to the pepper cytoplasmic male sterility restorer gene and its obtaining method and application
  • A molecular marker closely linked to the pepper cytoplasmic male sterility restorer gene and its obtaining method and application
  • A molecular marker closely linked to the pepper cytoplasmic male sterility restorer gene and its obtaining method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A molecular marker CaML closely linked with the pepper cytoplasmic male sterility restoration gene of this embodiment, said molecular marker is transformed from the genotyping primer designed at the SNP locus closely linked with the pepper cytoplasmic male sterility restoration gene .

[0046] The molecular marker CaML closely linked to the capsicum cytoplasmic male sterility restorer gene described above is obtained by the following method, including the following steps:

[0047] (1) Group construction:

[0048] The cytoplasmic male sterile line material YJ15(S / rfrf) was used as the female parent, and the restorer line materials XJ02(N / RfRf) and XJ07(N / RfRf) were used as the male parent to construct F2 segregating populations BL and HS, respectively.

[0049] (2) Phenotype identification:

[0050] F2 populations were planted in plastic greenhouses, covered with insect-proof nets, and managed according to the standardized production mode of pepper. By observing wheth...

Embodiment 2

[0058] Example 2 Verification of capsicum cytoplasmic male sterility restoration gene molecular marker CaML:

[0059] Utilize the recovery gene molecular marker primer that embodiment 1 obtains, respectively to the F in embodiment 1 2 Groups BL and HS are identified, and the steps are as follows:

[0060] (1) Using pepper genomic DNA as a template, the concentration is 80-120ng / μl; molecular marker CaML is used for PCR amplification, and the sequence of the forward primer is CaML-F: 5'-TGGCCTTGCAATCCGTTATG-3', and the sequence of the reverse primer is CaML-R: 5'-TGCTAGTGTTACGGGTATGCTC-3'. PCR reaction system: the total volume is 15 μl, and the specific components are as follows: ddH 2 O 10.4μl, 10×PCR Buffer 1.5μl, 1.2μl dNTPs (2mmol / L), 0.08μl Taq DNA polymerase (5U / μl), 0.4μl forward primer (10μM), 0.4μl reverse primer (10μM) and 1.0 μl DNA template.

[0061] (2) PCR amplification program: pre-denaturation at 95.0°C for 3 min; denaturation at 95.0°C for 30 s, renaturatio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention relates to a molecular marker closely linked with pepper cytoplasmic male sterility restorer gene and its obtaining method and application. The F2 segregation population HS is constructed by using the cytoplasmic male sterile line material YJ15 and the restorer line material XJ07; at the same time, YJ15 and Restorer material XJ02 constructed another F2 segregation population BL. Using extreme pooling combined with RNA-Seq and recombinant single-plant analysis to map the restorer genes in the HS population, and finally obtain the chromosomal regions and candidate genes that are closely linked to the restorer genes, and further design the molecular marker CaML based on the variation near the candidate genes. Using this marker to identify HS population, the coincidence rate reached 100%, and using this marker to identify BL population, the coincidence rate reached 93.2%. The invention provides tightly linked molecular markers for the cloning of the pepper cytoplasmic male sterility restorer gene and related breeding work, and has important utilization value.

Description

technical field [0001] The invention belongs to the fields of capsicum genetic breeding and molecular biology, and in particular relates to a molecular marker closely linked with a capsicum cytoplasmic male sterility restoration gene, an obtaining method and application thereof. Background technique [0002] Capsicum (Capsicum annuum L.) is an annual or perennial plant belonging to the Solanaceae Capsicum genus, which is widely planted all over the world and is an important vegetable crop. Pepper originated in South America, and its history of artificial domestication and cultivation is more than 6000 years. Around the late Ming Dynasty (16th century), pepper was introduced into my country. It has become an important part of the daily diet of people in my country, especially in the Yangtze River Basin. [0003] How to efficiently breed high-yield and high-quality pepper varieties has always been the concern of pepper breeders. The current pepper breeding in my country is s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6806C12N15/11
CPCC12Q1/6806C12Q1/6895C12Q2600/13
Inventor 欧阳波贾全锐于会洋周玉红刘峰陈文超李峰邹学校
Owner HUAZHONG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap