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SNP marker closely linked to tomato root-knot nematode resistance gene mi1 and its application

A tomato root-knot nematode and anti-root-knot nematode technology, which is applied in the directions of recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc. It can reduce the scale of field planting, reduce the cost of breeding, and shorten the detection period.

Active Publication Date: 2022-05-03
北京通州国际种业科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the breeding process of traditional root-knot nematode-resistant varieties, each generation needs to use the method of field inoculation to identify the genotype of the intermediate material, and the environmental conditions will affect the selection of the correct intermediate material, which will inevitably affect the breeding process; at the same time If the inoculation process is improperly operated, the pathogen will spread, which is not conducive to the comprehensive control of root-knot nematode disease. Therefore, the problem of difficult breeding of disease-resistant varieties has not been completely solved.
Although the above-mentioned Mi1 gene linkage markers that have been developed can be used for molecular marker-assisted selection, the detection efficiency is relatively low, and it is not suitable for high-throughput molecular detection platforms

Method used

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  • SNP marker closely linked to tomato root-knot nematode resistance gene mi1 and its application
  • SNP marker closely linked to tomato root-knot nematode resistance gene mi1 and its application
  • SNP marker closely linked to tomato root-knot nematode resistance gene mi1 and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0045] Example 1 Development of closely linked SNP markers with tomato root-knot nematode Mi1 gene

[0046] The development process of the SNP marker closely linked to the tomato root-knot nematode resistance Mi1 gene is as follows: figure 1 shown. According to literature reports, a SCAR marker primer pair Mi23F / Mi23R closely linked to the Mi1 gene was obtained (Gracia B E, Mejia L, SalusM S, et al. A co-dominant SCAR marker, Mi23, for detection of the Mi-1.2 gene for resistance to root-knot nematode in tomato germolasm [J]. Applied Mechanics and Materials, 2013, 486: 323-326).

[0047] 1. Determination of primers: The closely linked molecular marker Mi23 sequence of the Mi1 gene was anchored in the tomato chromosome 6 chromosome 2,320,942…2,320,969bp interval (Gracia B E, Mejia L, Salus M S, et al.A co-dominantSCAR marker, Mi23, for detection of the Mi-1.2 gene for resistance to root-knotnematode in tomato germolasm [J]. Applied Mechanics and Materials, 2013, 486: 323-326)....

Embodiment 2

[0073] Example 2 Application of SNP Marker Tightly Linked to Tomato Root Knot Nematode Resistance Gene Mi1 in Molecular Marker-Assisted Selection of Tomato Root Knot Nematode Resistance Plants

[0074] In order to detect the practicability of the KASP-Mi1 marker of the present invention, the tomato F1 variety material Zofia (Rickswan (China) Seed Co., Ltd.) was purchased, and according to the Zofia phenotype and genotype identification results, it was judged to be a root-knot Nematode heterozygous disease-resistant materials. 90 strains of F2 natural segregation population were produced by F1 natural selfing, and KASP marker detection and disease resistance phenotype verification were carried out on the segregation population (Table 2). The implementation methods of marker detection and disease resistance phenotype verification were the same as in Example 1. Through the detection and analysis of the phenotype and genotype of the segregation population, among the 90 individual ...

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Abstract

The invention discloses a SNP marker closely linked to tomato root-knot nematode resistance gene Mi1 and its application, and the primer combination of the SNP marker KASP-Mi1 developed based on KASP technology is: Primer X, Primer Y and Primer R. The present invention uses KASP technology to carry out rapid genotyping and identification of SNP sites closely linked to tomato root-knot nematode resistance gene Mi1, which can be applied in commercial molecular breeding with high, medium and low throughput; at the same time, it develops the SNP marker phenotype The selection efficiency reaches 96.9%, which can quickly and accurately detect the root-knot nematode resistance gene Mi1 in different germplasm resources of tomato; molecular marker-assisted selection can be carried out in the early stage of breeding, reducing the field planting scale of breeding groups and reducing breeding costs. Speed ​​up the breeding process.

Description

technical field [0001] The invention relates to the field of genome sequence and plant biotechnology, in particular to the marker of tomato root-knot nematode resistance gene Mi1 closely linked SNP site and its application method. Background technique [0002] Root-knot nematode is a kind of plant pathogenic nematode that threatens the world's agricultural production, causing economic losses of hundreds of billions of dollars in global crops every year. The control measures of root-knot nematode mainly adopt chemical control, biological control, agricultural measures and planting resistant varieties, etc. Among them, the breeding of tomato root-knot nematode-resistant varieties is one of the most effective and environmentally friendly methods to control tomato root-knot nematode disease. During the breeding process of traditional root-knot nematode-resistant varieties, each generation needs to use the method of field inoculation to identify the genotype of the intermediate m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/172
Inventor 刘宝平王均帅焦珍珍单云鹏霍秀爱尹增奇包格日乐
Owner 北京通州国际种业科技有限公司
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