Development and application of SNP markers for broad-spectrum blast resistance gene pigm in rice

Abstract

The present invention provides the development and application of a SNP marker of the broad-spectrum rice blast resistance gene Pigm, including the SNP molecular marker K_060508 closely linked with the rice blast resistance gene Pigm, and the SNP marker detects the 10421726 site of rice chromosome 6 The base is G or A, and the primer combination of the SNP tag developed based on KASP technology is Primer X, Primer Y and Primer C. The present invention uses KASP technology to rapidly genotype the SNP markers closely linked to the rice blast resistance gene Pigm, which can be applied in commercial molecular breeding with high, medium and low throughput; at the same time, the selection efficiency of developing SNP marker phenotypes reaches 100%, it can quickly and accurately detect the broad-spectrum rice blast resistance gene Pigm in different germplasm resources such as indica rice and japonica rice; it can carry out promising molecular marker-assisted selection in the early stage of breeding, reduce the field planting scale of breeding groups, and reduce the cost of breeding cost and speed up the breeding process.

Description

technical field [0001] The invention relates to the fields of molecular markers and crop breeding, in particular to the development and application of SNP markers of rice broad-spectrum rice blast resistance gene Pigm. Background technique [0002] Rice blast caused by Magnapothe oryzae is a worldwide rice planting disease, and the global rice yield loss due to rice blast can reach 11% to 30% every year. Long-term practice shows that the cultivation of disease-resistant varieties (line ) is currently the most economical and effective measure to control rice blast. In recent decades, with the development of molecular genetics, molecular marker-assisted selection has played a pivotal role in rice disease resistance breeding. The use of efficient molecular marker-assisted selection of single plants containing target genes for hybridization can accurately perform target traits. It can reduce the scale of field planting of selected breeding groups, shorten the breeding period, a...

AI Technical Summary

Problems solved by technology

However, most of the molecular markers used to detect Pigm are SSR, InDel or CAPS markers. These markers cannot effectively distinguish Pigm from other multiple alleles, and cannot completely distinguish Pigm donors and acceptors, and the detection results are likely to cause misjudgment.

In addition, the detection process of SSR, InDel or CAPS and other markers requires enzyme digestion, gel electrophoresis and other steps. The operation is complicated, the experimental cost is high, the detection throughput is small, and it is easy to pollute the environment and cause harm to the human body.

Images