Development and application of KASP molecular marker of rice blast resistance gene Pid3-A4
A rice blast disease and molecular marker technology, applied in the field of inductor rice breeding, can solve the problems of low detection efficiency, unsuitable high-throughput molecular detection platform, and aerosol pollution of the environment, so as to reduce pollution and reduce field planting. Scale, the effect of reducing breeding costs
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Embodiment 1
[0039] Example 1: Development of KASP marker closely linked to rice blast resistance gene Pid3-A4
[0040] The development process of the KASP marker closely linked to the rice blast resistance gene Pid3-A4 is as follows: figure 1shown. According to literature reports, a SNP locus [CA / AG] closely linked to the Pid3-A4 gene was obtained (the physical location of the rice reference genome is chromosome 6 10389855-10389856bp).
[0041] 1. Determination of primers:
[0042] The tightly linked SNP locus of Pid3-A4 gene was anchored at the 13058850bp position of rice chromosome 6 (Huang Weiheng, Huang Zhiyuan, Tang Li, et al. Development and application of specific InDel molecular markers for rice blast resistance Pid3 / Pid3-A4 gene[J] . Hybrid Rice, 2020(2):68-74.). The flanking sequence of closely linked SNP sites of Pid3-A4 gene was downloaded from NCBI database, and three sets of KASP primers were designed using Primer5.0 software. After detection by the ArrayTape platform of...
Embodiment 2
[0070] Example 2: Application of KASP marker closely linked to rice blast resistance gene Pid3-A4 in molecular marker-assisted selection of rice blast resistance plants
[0071] see image 3 , in order to detect the practicability of the KASP-A4 primer of the present invention, the F1 population was obtained by crossing the cultivated rice blast-resistant material 311100 with the rice blast-susceptible material Daohuaxiang No. 2, and 90 F2 natural segregating populations were produced by natural selfing of F1. For the segregating population Carry out KASP marker detection and disease-resistant phenotype verification (Table 2), the implementation method of marker detection and disease-resistant phenotype verification refers to Example 1. Through phenotypic and genotypic detection and analysis of segregating populations (e.g. image 3 shown), among the 90 isolated populations, only 6 genotypes were inconsistent with the phenotype results, and the consistency result between the ...
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