Development and application of KASP marker of rice grain type gene GLW7
A technology of rice and grain type, applied in the direction of microbial measurement/inspection, biochemical equipment and methods, etc., to speed up the breeding process, reduce pollution, and reduce breeding costs
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Embodiment 1
[0034] Development of KASP Marker with Rice Grain Shape Gene GLW7
[0035] The process of developing KASP markers with the rice grain shape gene GLW7 is as follows: figure 1 As shown, according to literature reports, a SNP locus closely linked with GLW7 gene was obtained.
[0036] 1. Determination of primers: The tightly linked SNP site of GLW7 gene was anchored at the position of 19103121bp on rice chromosome 7 (Liang Wenhua, Zhang Yadong, Wang Cailin, et al. Development of rice GLW7 gene functional markers and gene effect analysis[J]. Journal of Jiangsu Agricultural Science, 2020,036(002):257-264.), downloaded the flanking sequence of closely linked SNP sites of GLW7 gene from NCBI database, and used Primer5.0 software to design 3 sets of KASP primers, and used Douglas Scientific After detection by the company's ArrayTape platform, a set of KASP primers with good polymorphisms was selected for subsequent verification. The primers used to detect KASP markers closely linked t...
Embodiment 2
[0061] Application of KASP Marker with Rice Grain Type Gene GLW7 in Molecular Marker-Assisted Selection of Long Grain Type Rice Plants
[0062] In order to test the practicability of the KASP_GLW7 site of the present invention, the F1 population was obtained by crossing the long-grain material Yangjing 805 with the short-grain material Shennong 265, and 48 F2 natural segregation populations were produced by natural selfing of F1, and KASP was performed on the segregation population. Marker detection and grain type phenotype verification (table 2), marker detection and grain type phenotype verification implementation method reference example 1, phenotype and genotype detection and analysis are carried out to the isolated population, the genotype is the average grain size of a single plant of CC length is 9.39, the average grain length of a single plant with genotype GG is 7.81, and the average grain length of a single plant with genotype CC is significantly higher than that of a...
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