Development and application of KASP marker of rice grain type gene GLW7

A technology of rice and grain type, applied in the direction of microbial measurement/inspection, biochemical equipment and methods, etc., to speed up the breeding process, reduce pollution, and reduce breeding costs

Inactive Publication Date: 2022-06-10
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although SSR markers, InDel markers or enzyme-cut markers can be used for molecular marker-assisted selection, the detection efficiency is low, and aerosol pollution may be generated at the same time, which is not suitable for high-throughput molecular detection platforms

Method used

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  • Development and application of KASP marker of rice grain type gene GLW7
  • Development and application of KASP marker of rice grain type gene GLW7
  • Development and application of KASP marker of rice grain type gene GLW7

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Experimental program
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Effect test

Embodiment 1

[0034] Development of KASP Marker with Rice Grain Shape Gene GLW7

[0035] The process of developing KASP markers with the rice grain shape gene GLW7 is as follows: figure 1 As shown, according to literature reports, a SNP locus closely linked with GLW7 gene was obtained.

[0036] 1. Determination of primers: The tightly linked SNP site of GLW7 gene was anchored at the position of 19103121bp on rice chromosome 7 (Liang Wenhua, Zhang Yadong, Wang Cailin, et al. Development of rice GLW7 gene functional markers and gene effect analysis[J]. Journal of Jiangsu Agricultural Science, 2020,036(002):257-264.), downloaded the flanking sequence of closely linked SNP sites of GLW7 gene from NCBI database, and used Primer5.0 software to design 3 sets of KASP primers, and used Douglas Scientific After detection by the company's ArrayTape platform, a set of KASP primers with good polymorphisms was selected for subsequent verification. The primers used to detect KASP markers closely linked t...

Embodiment 2

[0061] Application of KASP Marker with Rice Grain Type Gene GLW7 in Molecular Marker-Assisted Selection of Long Grain Type Rice Plants

[0062] In order to test the practicability of the KASP_GLW7 site of the present invention, the F1 population was obtained by crossing the long-grain material Yangjing 805 with the short-grain material Shennong 265, and 48 F2 natural segregation populations were produced by natural selfing of F1, and KASP was performed on the segregation population. Marker detection and grain type phenotype verification (table 2), marker detection and grain type phenotype verification implementation method reference example 1, phenotype and genotype detection and analysis are carried out to the isolated population, the genotype is the average grain size of a single plant of CC length is 9.39, the average grain length of a single plant with genotype GG is 7.81, and the average grain length of a single plant with genotype CC is significantly higher than that of a...

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Abstract

The invention discloses development and application of a KASP marker of a rice grain type gene GLW7, and belongs to the technical field of genome sequences and plant biology. The method comprises the following steps: detecting the genotype of to-be-detected rice, and identifying or assisting in identifying the rice grain type according to the genotype of the to-be-detected rice, the genotype is the genotype of a KASPGLW7 site in a rice genome; the KASPGLW7 site is an SNP site in a rice genome, the nucleotide type of the KASPGLW7 site is C or G, and the KASPGLW7 site is the 101st nucleotide of SEQ ID No.4 in a sequence table. According to the invention, the KASP technology is utilized to carry out rapid gene typing identification on the SNP locus closely linked with the rice grain type gene GLW7, and the SNP locus can be applied to commercial molecular breeding with high, medium and low throughput; meanwhile, the molecular marker KASPGLW7 is relatively high in grain length phenotype selection efficiency, and the rice grain type gene GLW7 can be rapidly and accurately detected in different germplasm resources of rice; molecular marker-assisted selection can be carried out in the early breeding stage, the breeding cost is reduced, and the breeding process is accelerated.

Description

technical field [0001] The invention relates to the field of genome sequence and plant biotechnology, in particular to the development and application of KASP marker of rice grain type gene GLW7. Background technique [0002] Rice (Oryza sativa) is one of the most important food crops in my country, and more than 60% of the population in China takes rice as a staple food. In 2020, my country's rice planting area will reach 450 million mu, accounting for about a quarter of the total grain area. In addition to food, rice can also be used as alcohol raw material, feed, fuel and packaging materials. [0003] Grain shape is one of the important indicators of rice, and it is an important factor affecting rice yield and quality. Rice with longer grain shape tends to have higher yield (Zhang Lin, Liu Yi, Zhang Siwei, etc. The density of rice and thousand-grain weight and grain shape Correlation study of traits [J]. China Rice, 2019, 25(01): 83-86.). Therefore, cultivating long-gr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2563/107C12Q2531/113
Inventor 许作鹏张宏根陈志爱汤述翥
Owner YANGZHOU UNIV
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