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A method and application of using ems mutagen to create red kidney bean mutants

A technology for red kidney beans and mutants, applied in the fields of application, botanical equipment and methods, plant genetic improvement, etc., can solve problems such as health threats to breeders, and achieve the goal of reducing health threats, reducing dosage, and improving mutagenesis efficiency. Effect

Inactive Publication Date: 2021-08-03
CORN RES INST SHANXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] EMS is a commonly used plant mutagen. EMS belongs to a kind of alkylating agent and has high mutagenic efficiency. In the prior art, the usual volume fraction of EMS mutagen is 0.1-1.0%, but this substance has strong carcinogenicity and Volatile, when used, strict protection must be done, otherwise there will be a serious threat to the health of breeders

Method used

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  • A method and application of using ems mutagen to create red kidney bean mutants
  • A method and application of using ems mutagen to create red kidney bean mutants

Examples

Experimental program
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Effect test

Embodiment 1

[0037] A kind of method adopting EMS mutagen to create red kidney bean mutant, comprises the following steps:

[0038] S1, seed pretreatment

[0039] Select red kidney bean seeds with consistent color, full grain and no insects to carry out disinfection treatment, and then soak in 20mg / L gibberellin solution for 3 hours to obtain sterile seeds;

[0040] S2, mutagenesis treatment

[0041] Aseptic seeds are alternately soaked in the EMS (ethyl methanesulfonate) solution of plant growth regulator and volume fraction 0.01%, wherein the soaking time of plant growth regulator is 10min, and the soaking time of EMS solution is the plant growth regulator Twice of that; the number of times of alternation is 2 times (i.e. plant growth regulator 10min-EMS solution 20min-plant growth regulator 10min-EMS solution 20min); dry the seed surface moisture to obtain the first-grade mutagenic seeds;

[0042] The plant growth regulator is cytokinin (N6-isopentenyl adenine), and the concentration ...

Embodiment 2

[0047] A kind of method adopting EMS mutagen to create red kidney bean mutant, comprises the following steps:

[0048] S1, seed pretreatment

[0049] Select red kidney bean seeds with consistent color, full grain and no insects to carry out disinfection treatment, and then soak in 30mg / L gibberellin solution for 2 hours to obtain sterile seeds;

[0050]S2, mutagenesis treatment

[0051] Aseptic seeds are alternately soaked in the EMS (ethyl methanesulfonate) solution of plant growth regulator and volume fraction 0.05%, wherein the soaking time of plant growth regulator is 5min, and the soaking time of EMS solution is plant growth regulator twice; the number of alternations is 5 times (that is, plant growth regulator 5min-EMS solution 10min-plant growth regulator 5min-EMS solution 10min-plant growth regulator 5min-EMS solution 10min-plant growth regulator 5min-EMS solution 10min -Plant growth regulator 5min-EMS solution 10min); Blot the moisture on the surface of the seeds to...

Embodiment 3

[0057] A kind of method adopting EMS mutagen to create red kidney bean mutant, comprises the following steps:

[0058] S1, seed pretreatment

[0059] Select red kidney bean seeds with consistent color, full grain and no insects to carry out disinfection treatment, then soak in 25mg / L gibberellin solution for 2.5h to obtain sterile seeds;

[0060] S2, mutagenesis treatment

[0061] Aseptic seeds are alternately soaked in the EMS (ethyl methanesulfonate) solution of plant growth regulator and volume fraction 0.02%, wherein the soaking time of plant growth regulator is 8min, and the soaking time of EMS solution is plant growth regulator double; Alternate number of times is 3 times (i.e. plant growth regulator 8min-EMS solution 16min-plant growth regulator 8min-EMS solution 16min-plant growth regulator 8min-EMS solution 16min); Blot the seed surface moisture to obtain a grade mutagenic seeds;

[0062] Described plant growth regulator is 6-benzylaminopurine, and the concentratio...

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Abstract

The invention belongs to the technical field of kidney bean breeding, and in particular relates to a method and application of using an EMS mutagen to create red kidney bean mutants. Including seed pretreatment, mutagenesis treatment and sowing cultivation, wherein the mutagenesis treatment step is: alternately soak the sterile seeds in the EMS solution with a volume fraction of 0.01-0.05% of the plant growth regulator, wherein the soaking time of the plant growth regulator For 5-10min, the soaking time of the EMS solution is twice that of the plant growth regulator; the number of times of alternation is 2-5 times; the seed surface moisture is blotted to obtain the first-level mutagenic seeds; the first-level mutagenic seeds are obtained with ray radiation Secondary mutagenesis seeds; sowing and cultivating, screening out red kidney bean mutants. The method of the present invention is a special high-efficiency mutagenesis method for red kidney beans, which improves the mutagenesis efficiency, reduces the consumption of carcinogens such as EMS, and reduces the health threat to breeders.

Description

technical field [0001] The invention belongs to the technical field of kidney bean breeding, and in particular relates to a method and application of using an EMS mutagen to create red kidney bean mutants. Background technique [0002] Red kidney bean is an angiosperm of the leguminous family. It has large grains and bright color. It has both nutritional and medicinal values, and is nutritious and healthy. Red kidney beans are rich in anthocyanins and saponins, which can reduce the content of local inflammatory tissues in joints, have obvious anti-inflammatory effects, and have anti-inflammatory and analgesic effects on arthritis patients. Red kidney beans are a specialty product in Shanxi, my country. The planting area of ​​red kidney beans in Kelan County reaches 130,000 mu, and the red kidney beans can be exported to earn 3.5 million US dollars, which greatly increases farmers' income. Maintaining the quality of the existing red kidney beans and developing new red kidney...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 陈喜明韩云丽邵林生郭贵青李晓峰
Owner CORN RES INST SHANXI ACAD OF AGRI SCI
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