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A method for efficiently creating new rhododendron germplasm by combining distant hybridization and tissue culture

A tissue culture and distant hybridization technology, applied in the fields of botanical equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of long breeding cycle, poor rooting, restricting the use of hybrid azaleas, etc., to shorten the breeding cycle, reduce The difficulty of cultivation management and the effect of shortening the breeding cycle

Active Publication Date: 2019-08-06
WUHAN ACADEMY OF AGRI SCI
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In the prior art, when rhododendrons are used for hybrid breeding, they often pay attention to the early work of zygotic embryo formation, such as parent selection, but lack of systematic research on the later work, which limits the use of rhododendron hybrids, especially distant hybrids between species, in new germplasm resources. The use of creative aspects; these factors are not conducive to the genetic breeding work of rhododendron interspecific hybrids
Therefore, a method to achieve distant hybridization and tissue culture of Rhododendron is obtained through research to solve the problems of poor seed germination rate, weak seedling formation, poor rooting, and long breeding cycle of Rhododendron interspecific hybrids, and to achieve excellent quality of Rhododendron. The rapid and efficient aseptic culture of germplasm can transfer the excellent stress resistance traits of spring azalea varieties into rhododendron ornamental varieties, effectively improve the germplasm of rhododendrons, and enrich the germplasm resources of wild rhododendrons in my country. The genetic basis of Rhododendron varieties and optimized breeding technology play an important and positive role, which is particularly important for the original breeding work of Rhododendron and the sustainable development of the industry

Method used

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  • A method for efficiently creating new rhododendron germplasm by combining distant hybridization and tissue culture
  • A method for efficiently creating new rhododendron germplasm by combining distant hybridization and tissue culture
  • A method for efficiently creating new rhododendron germplasm by combining distant hybridization and tissue culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Hybridization test of 'Rouge honey' × 'Chunwubao'

[0034] According to the results of the pollen viability test ( figure 1 ) and stigma receptivity studies ( figure 2 ) and so on, to determine the final parental combination, wherein the male parent of the hybrid is the Dongjuan series variety 'Yanzhimi', and the female parent is the Chunjuan series variety 'Chunwubao', and they are used as materials for hybridization experiments. The specific process is to select large flower buds in the balloon-like stage, emasculate and bag the female parent before flowering, collect fresh pollen from the male parent, and carry out routine artificial pollination at 9:00-12:00 on a sunny day. Repeat each combination for at least 20 days. After pollination, continue to bag and hang tags. After pollination, put a sulfuric acid paper bag on the female parent, seal the bag opening, and mark the parent’s name and hybridization date; collect rhododendron hybrid capsules from mi...

Embodiment 2

[0035] Embodiment 2: Different disinfection treatment tests of rhododendron hybrid seeds

[0036] Get the capsule that embodiment 1 hybridization test obtains, it is peeled off in ultra-clean workbench, and obtain the seed that is pure without impurity, after seed is through the different types of disinfection as shown in table 1, be spread on germination medium ( 1 / 2WPM medium+GA 3 100mg / L, pH=5.6) to carry out germination culture, put the petri dish in a constant temperature incubator with a temperature of 24-26°C, a relative humidity of 65-75%, and a photoperiod of 16 / 8h for cultivation, and successfully grow seedlings , and then transfer the seedlings to the rooting medium and continue to cultivate them into Rhododendron aseptic tissue culture seedlings about 8-10 cm high.

[0037] Table 1 Design table of different disinfection treatments for rhododendron hybrid seeds

[0038]

[0039] Each treatment was replicated three times, and 100 seeds were inoculated in each re...

Embodiment 3

[0042] Embodiment 3: Screening of medium for tissue culture of rhododendron hybrid seeds

[0043] The rhododendron hybrid seeds obtained in the hybridization test in Example 1 were treated with 1.5% NaClO solution for 10 minutes, soaked in 75% ethanol for 30 seconds, and then washed 2-3 times with sterile water. After 5 weeks of growth, the growth status of the germinated seedlings (leaf color, plant height, root length) was classified and counted. The selected medium is: 1 / 2MS, MS, 1 / 2WPM, WPM, and H 2 O was used as a blank control medium.

[0044] As shown in Table 2, the four mediums are all beneficial to the growth of germinated seedlings to a certain extent, and the WPM medium is obviously better than the MS medium. At the same time, 1 / 2WPM medium culture can stably and efficiently promote the growth of seedlings in terms of leaf color, plant height and root length, so we can conclude that 1 / 2WPM medium is the most suitable medium for aseptic sowing of Rhododendron.

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Abstract

The invention provides a method for creating new azalea germplasm by combining distant hybridization and tissue culture at high efficiency. The method comprises the following steps of using Dongjuan series variety (Yanzhimi) as a male parent, using Chunjuan series variety (Chunwubao) as a female parent, hybridizing, collecting the capsule after hybridizing at the current year, stripping seeds, developing a sterile seedling with 7 to 10 buds and height of about 8 to 10cm by a special tissue culture technique, and performing subculture rooting, so as to obtain four to six times of propagation coefficient. Compared with the traditional field cuttage or grafting propagation (20 or less of propagation coefficient within one year), the method can greatly shorten the breeding cycle of the novel variety.

Description

technical field [0001] The invention belongs to the technical field of plant hybridization and breeding, in particular, relates to a method for distant hybridization and tissue culture of plants to select and breed new germplasm, in particular to a method for efficiently creating new rhododendron germplasm by combining distant hybridization and tissue culture . Background technique [0002] Rhododendron spp. is a plant of the Rhododendron family Rhododendron genus, commonly known as azalea, and is one of China's "Ten Traditional Famous Flowers". And very rich garden application value. China is the origin and diversified distribution center of Rhododendron. There are nearly 571 species of wild resources in the distribution, accounting for nearly 2 / 3 of the wild Rhododendron germplasm resources in the world. These wild germplasms have great variations in ornamental and adaptability , is a germplasm resource worthy of wide use. [0003] Due to the introduction of wild resour...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H4/00
CPCA01H1/02A01H4/001A01H4/008
Inventor 方林川周媛童俊徐冬云毛静董艳芳杨婧瑜陈卫东
Owner WUHAN ACADEMY OF AGRI SCI
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