Protein capable of controlling weight of crop seeds and application thereof

A protein and crop technology, applied in the field of plant genetics and breeding, can solve problems such as difficulty, small contribution rate of grain weight, and difficulty in fine positioning

Active Publication Date: 2018-09-04
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The inheritance of corn kernel weight is mainly based on additive effects, and kernel weight is jointly controlled by multiple minor genes. The effect value of a single QTL is relatively small, and the contribution rate to kernel weight is small, making fine positioning relatively difficult. It is difficult to directly apply the main effect QTL related to grain weight to breeding practice

Method used

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  • Protein capable of controlling weight of crop seeds and application thereof
  • Protein capable of controlling weight of crop seeds and application thereof
  • Protein capable of controlling weight of crop seeds and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Mapping of maize qHKW1-9 gene

[0045] Zheng 58 and SK are two middle inbred lines in maize. Zheng 58 has large ears, many grains, large and heavy grains, and high yield, while SK has small ears, few grains, small and light grains, and low yield ( figure 1 A, B). Therefore, by constructing the recombinant inbred line (RIL) population of Zheng 58×SK, the traits of maize kernels were initially mapped, and it was found that there is a major QTL on the short arm of chromosome 1 that can affect the grain weight of the grain (grain weight as 100-kernel weight trait index measurement)( figure 1 C), name this QTL qHKW1. According to the results of multi-point location for many years, this QTL can be detected in 7 environmental points affecting 100-grain weight ( figure 1 C), the candidate interval of this QTL is 65-77cM, and the length of the corresponding physical location interval is 16Mb. At different environmental points, the maximum LOD (limit of detection) v...

Embodiment 2

[0053] Example 2 qHKW1-9 protein function and its identity protein

[0054] Analysis of the protein sequence encoded by the qHKW1-9 gene revealed that the gene encodes a total of 1 amino acid (sequence shown in SEQ ID NO.1), which belongs to leucine-rich repeat receptor-like serine / threonine-protein kinase protein. Further use of the BLASTP tool in the NCBI database (https: / / www.ncbi.nlm.nih.gov / ) found that the qHKW1-9 protein has identity proteins in other plants, and the sequence similarity between these proteins and the qHKW1-9 protein Extremely high and all belong to leucine-rich repeat receptor-like serine / threonine-protein kinase type proteins. These proteins come from sorghum, millet, millet, japonica rice, indica rice, wild rice with short anthers, barley, barley, Brachypodium distachys and other plants, and their amino acid sequence similarity with qHKW1-9 protein is at least 81%, 82% %, 83%, 84%, 85%, 89% or 92% (Table 2), the amino acid sequence is shown in SEQ ID...

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Abstract

The invention discloses a protein capable of controlling weight of crop seeds and application thereof, and belongs to the field of foliage genetic breeding. The protein is qHKW1-9 protein in corn, andthe amino acid sequence is shown as SEQ ID NO.1, or the amino acid sequence contains protein with at least 81%, at least 82%, at least 83%, at least 85%, at least 89% or at least 92% of sequence similarity relative to the sequence in SEQ ID NO.1. The invention further discloses nucleic acid for encoding the abovementioned protein, an expression cassette, an expression carrier and a host cell containing the nucleic acid, and application thereof in increasing of the weight of the crop seeds.

Description

technical field [0001] The invention relates to a protein for controlling grain weight of crops and an application thereof, belonging to the technical field of plant genetics and breeding. Background technique [0002] The high yield of maize is the result of the coordinated development of the three yield factors per unit area, the number of grains per ear, and the grain weight. Among them, the grain weight is the last factor that determines the yield, and the reduction of the grain weight cannot be compensated by the other two yield factors. Therefore, it has significance in high-yield breeding. Therefore, the analysis of the genetic mechanism of corn kernel weight not only helps to understand the genetic basis of maize yield, but also has important practical significance for the genetic improvement of maize high yield. [0003] The genetic basis of corn kernel weight traits has attracted much attention. Although many scientists at home and abroad have conducted a large nu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/10A01H6/46
CPCC12N15/8261C07K14/415
Inventor 刘杰严建兵
Owner HUAZHONG AGRI UNIV
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