Method for enriching high density anaerobic ammonia oxidizing bacteria in sewage nitrogen removing system

Abstract

The invention discloses a method for enriching high density anaerobic ammonia oxidizing bacteria in a sewage nitrogen removing system. The method comprises following steps: (1) collecting nitrification sludge that contains floras of anaerobic ammonia oxidizing bacteria, preparing an active sludge solution, inoculating the solution to an enrichment medium, and carrying out nitrification domestication until the ammonia nitrogen conversion rate is not less than 80%; (2) inoculating the domesticated sludge to a reactor, adding a macroporous polyurethane filler, matrix ammonia nitrogen, and nitritenitrogen into the reactor, and starting the anaerobic ammonia oxidizing reactor at a low matrix concentration; (3) increasing the addition amount of matrix, and starting the anaerobic ammonia oxidizing reactor at a medium-high matrix concentration; and (4) increasing the addition amount of matrix, and enriching the high density anaerobic ammonia oxidizing bacteria. The macroporous polyurethane filler can provide a micro-aerobic environment for anaerobic ammonia oxidizing bacteria and can adapt to the agglomeration growth characteristics of anaerobic ammonia oxidizing bacteria; bacteria such as AOB, NOB, etc. can be eliminated, and high purity anaerobic ammonia oxidizing bacteria with high abundance can be obtained.

Description

Technical field [0001] The invention belongs to the technical field of environmental engineering microorganisms, and specifically relates to a method for enriching high-density anammox bacteria in a sewage denitrification treatment system. Background technique [0002] A large amount of ammonia nitrogen discharged into the water body will cause eutrophication of the water body. At present, ammonia nitrogen has become a binding index for total pollutant control, so the removal of nitrogen in the sewage treatment process has attracted more and more attention. However, the traditional denitrification technology has the disadvantages of high energy and material consumption, long process flow, and a large amount of organic carbon sources. Therefore, the development of denitrification technology with low energy and material consumption has become a research focus in the field of water pollution control. [0003] The discovery of the ANAMMOX phenomenon is a major discovery in the field o...

AI Technical Summary

Problems solved by technology

[0005] There are many difficulties in the enrichment of anammox bacteria. For example, the generation cycle of anammox bacteria is as long as 11 days, and the cell yield of ANAMMOX bacteria is 0.066mol C / mol NH 4 + , the external environmental conditions are very harsh. So far, people can only analyze ANAMMOX bacteria by analyzing the mixed culture of ANAMMOX bacteria, and cannot obtain ANAMMOX strains through pure culture. Anammox bacteria have a group sensing effect and must be enriched to a certain level. Abundance works

Images