The invention relates to a culture method of an artificial cordyceps militaris fruiting body. The culture method comprises the following steps of (1), culture medium preparation, specifically, mixingrice, soybean powder, water, tomato juice and an inorganic salt, and carrying out moist heat sterilization to form a culture medium; (2), hypha inoculation; (3), dark culture; (4), illumination culture, specifically, after white hyphae grow on the culture medium subjected to dark culture, putting a culture room under an illumination condition for culture, and gradually generating the cordyceps militaris fruiting body; and (5), cordyceps militaris fruiting body collection, specifically, collecting the cultured cordyceps militaris fruiting body. Compared with the prior art, the cordyceps militaris fruiting body cultured by the method is high in yield, cordycepin content, cordyceps polysaccharide content and adenosine content, and then the cordyceps militaris fruiting body has huge value in the fields of tumor resistance, bacteria resistance, virus resistance, anoxia resistance, fatigue resistance, cardiopulmonary regulation and the like.