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Needle mushroom liquid strain preparation method

A technology for liquid strains and Flammulina velutipes, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, fungi, etc., to achieve the effects of simple method, rapid growth, and large production volume.

Inactive Publication Date: 2018-09-11
HUAIHAI INST OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The industrial production process of Flammulina velutipes in my country has gradually formed a model, but compared with the international developed level, there is still a big gap

Method used

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  • Needle mushroom liquid strain preparation method
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  • Needle mushroom liquid strain preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1, a kind of preparation method of Flammulina velutipes liquid bacterial classification, its steps are as follows:

[0030] (1) Inoculate Flammulina velutipes mother strains on PDA plate medium, and incubate at 23°C for 6 days until the colonies cover the entire plate; the PDA medium formula is: 200 g of potatoes, 20 g of glucose, 15 g of agar, and 1000 mL of water;

[0031] (2) Use a puncher to select bacteria blocks from the plate, inoculate them into the seed medium, inoculate at 5% (w / v), rotate at 120 r / min, and incubate at 20°C for 6-7 days. This is the seed solution; The formula of the seed medium is: white granulated sugar 30 g, soybean protein concentrate 15 g, potassium dihydrogen phosphate 1 g, magnesium sulfate 0.5 g, water 1000 mL, pH 6.0-6.7;

[0032] (3) Using Soybean milk treasure fermentation medium, inoculate the seed solution into a pneumatic fermenter with an inoculum size of 1% (v / v), culture with aeration and stirring at a pressure of 0....

Embodiment 2

[0035] Embodiment 2, a kind of preparation method of Flammulina velutipes liquid spawn, its steps are as follows:

[0036] (1) Inoculate Flammulina velutipes mother strains on PDA plate medium and culture at 23 °C for 7 days until the colonies cover the entire plate; the PDA medium formula is: 200 g of potatoes, 20 g of glucose, 15 g of agar, and 1000 mL of water;

[0037] (2) Use a puncher to select bacterial blocks from the plate, inoculate them into the seed medium, inoculate the amount of 8% (w / v), rotate at 160 r / min, and incubate at 20°C for 7 days. This is the seed liquid; The basic formula is: white granulated sugar 40 g, soybean protein concentrate 20 g, potassium dihydrogen phosphate 1 g, magnesium sulfate 0.5 g, water 1000 mL, pH 6.0-6.7;

[0038] (3) Using Soybean milk treasure fermentation medium, inoculate the seed solution into a pneumatic fermenter with an inoculum size of 2% (v / v), aerate and stir the culture, the aeration pressure is 0.03 MPa, and cultivate at ...

Embodiment 3

[0041] Embodiment 3, a kind of preparation method of Flammulina velutipes liquid spawn, its steps are as follows:

[0042] (1) Inoculate Flammulina velutipes mother strains on PDA plate medium, culture at 23 °C for 6 days until the colonies cover the entire plate; the PDA medium formula is: 200 g of potatoes, 20 g of glucose, 15 g of agar, and 1000 mL of water;

[0043] (2) Use a puncher to select bacterial blocks from the plate, inoculate them into the seed medium, the inoculum amount is 5% to 8% (w / v), the rotation speed is 140 r / min, and cultivate at 20°C for 6 days. This is the seed solution ;The formulation of the seed medium is: white granulated sugar 35 g, soybean protein concentrate 18 g, potassium dihydrogen phosphate 1 g, magnesium sulfate 0.5 g, water 1000 mL, pH 6.0-6.7;

[0044] (3) Using Soymilk Bao fermentation medium, inoculate the seed solution into a pneumatic fermenter with an inoculum size of 1% to 2% (v / v), culture with aeration and stirring at a pressure ...

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Abstract

The invention discloses a needle mushroom liquid strain preparation method which comprises the following steps: taking needle mushroom mother strain, inoculating a PDA culture medium with the needle mushroom mother strain, choosing a strain block, inoculating a seed culture medium with the strain block and inoculating a pneumatic type fermentation tank with a seed solution by utilizing a soycomilfermentation medium or inoculating the pneumatic type fermentation tank with the seed solution by utilizing a compound nitrogen source fermentation medium to obtain the needle mushroom liquid strain.The needle mushroom liquid strain preparation method disclosed by the invention has the advantages of manure technology, large production, high automation degree and ability in saving labor sources and reducing production cost; a pollution rate is obviously reduced, a nutritional ingredient content in the fermentation medium is lower, and a culture temperature is also low; the liquid strain obtained under the conditions is more suitable for cultivation material environment, hypha restores more quickly after a cultivation material is inoculated, and strain culturing and mushroom producing timeis beneficial to be shortened.

Description

technical field [0001] The invention relates to a preparation method of strains, in particular to a preparation method of liquid strains of Flammulina velutipes. Background technique [0002] Flammulina velutipes ( Flammulina velutipes ), also known as winter mushroom, portobello mushroom, hairy-stalked money fungus, etc., is a well-known edible and medicinal fungus. At present, Flammulina velutipes has become one of the main edible fungi artificially cultivated in my country; in the industrial production of edible fungi, Flammulina velutipes is the edible fungus with the highest degree of industrialization and the largest output. [0003] With the increase of market demand for edible fungi, the cultivation technology and production process of Flammulina velutipes have been improved unprecedentedly, and the scale of factoryization is also constantly expanding. Compared with traditional solid strains, liquid strains have obvious advantages, such as easy dispersion, fast ger...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 李福后王伟霞刘伟
Owner HUAIHAI INST OF TECH
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