Method for analyzing insect semiochemicals by combining GC-MS and electroantennography

Abstract

The invention provides a method for analyzing insect semiochemicals by combining GC-MS and an electroantennography and belongs to the technical field of screening and determination of compounds. According to the method, screening of the insect semiochemicals and identification of sample material components can be synchronously performed, experiment steps are simplified, quick and efficient, the consistency of experimental data can be ensured, and higher economy and practicality are realized. The technical scheme is that the method comprises the following steps: cutting off tentacles of insectsalong base parts, removing small parts of top ends, then respectively fixing two ends of in vitro tentacles on a probe of the electroantennography by using a conductive adhesive, and enabling the tentacles to be connected with a microelectrode; feeding a sample into a gas chromatograph, and carrying out GC-MS and an electroantennography combined analysis; carrying out mass spectrometric detectionon peaks appearing at the same retention time of a GC-MS chromatogram and an electroantennography chromatogram, and identifying the insect semiochemicals with the activity by checking a standard spectral library with a standard mass spectrogram of the compounds. The method provided by the invention can be applied in a sensory mechanism of the insects to the semiochemicals, screening of active pheromone components or secondary plant substances and the like.

Description

technical field [0001] The invention belongs to the technical field of compound screening and determination, and in particular relates to a method for analyzing insect semiochemical substances by combining GC-MS and an antenna potentiometer. Background technique [0002] With the rapid development of my country's economy, people's living standards are improving day by day, especially the continuous improvement of the demand for high-quality daily diet and living environment, which has given birth to the rapid development of green agriculture and organic agriculture. In the process of crop production, it is inevitable Facing the problem of agricultural pests, therefore, people are forced to look for green prevention and control techniques to prevent and control pests. The use of insect semiochemicals to inhibit pest damage can not only reduce the loss caused by pest damage, but also protect the ecological environment, ensure the quality and safety of crops, and promote the ove...

AI Technical Summary

Problems solved by technology

GC-EAD has many successful cases in the study of insects and semiochemicals, but it needs to be used with GC-MS, and under the same chromatographic conditions, the qualitative steps of biologically active components are cumbersome, and the output of the two is cumbersome. Peak time will vary

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