A kind of large-scale propagation method of the red pine leaf spot borer biting wasp
A technology of Spotted Spot Borer and Spotted Spotted Borer, which is applied to Spotted Spotted Spot Borer, large-scale propagation, and control of Spotted Spotted borer, and can solve the problem of lack of pests, low drug resistance, pest outbreaks, etc. problem, to achieve the effect of easy operation
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specific Embodiment approach 1
[0033] Embodiment 1: The cultivation method of the Korean pine borer wasp in this embodiment includes the following steps:
[0034] 1, bee seed collection and preservation: collecting and identifying bee seeds in pupal stage of the fruit-tip borer, and preserving at low temperature;
[0035]Step 2, reproduction of bee species: taking out the low-temperature preserved Rhinophylla koraiensis, inoculating 2-30 Rhinophylla koraiensis into each host pupa, and putting the host pupa parasitized by Rhinophylla koraiensis into a constant temperature box or a greenhouse for cultivation.
[0036] In the second step, before receiving bees, the host pupa is soaked in hypochlorous acid aqueous solution with a concentration of ≤ 25%, then taken out, washed with distilled water and dried.
[0037] The functional relationship curve between the development duration and temperature of the red-tip borer is as follows Figure 1 Shown.
[0038] Hypochloric acid can soften the cuticle of pupae, especiall...
specific Embodiment approach 2
[0040] Embodiment 2: The difference between this embodiment and Embodiment 1 is: in Step 2, the effective accumulated temperature for breeding the stichopus koraiensis is 1-3 days of female bee day, and the humidity is 50-75%. Other steps and parameters are the same as the first embodiment.
[0041] Table 1 shows the starting temperature and effective accumulated temperature of the development of Rhinophylla koraiensis (with Rhinophylla koraiensis as the host).
[0042] Table 1
[0043]
specific Embodiment approach 3
[0044] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that in Step 2, the biting wasp of the fruit-tip borer is a female bee that emerged on the same day or 1-2 days after emergence. Other steps and parameters are the same as those of Embodiment 1 or 2.
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