Bone targeting salmon calcitonin and preparation method thereof
A salmon calcitonin and bone-targeting technology, which is applied in the field of biomedicine, can solve the problems of reduced biological efficacy, achieve the effects of less difficult operation, easy production and application, and improved bioavailability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0037] In this embodiment, a method for preparing bone-targeted salmon calcitonin is provided, and the steps are as follows:
[0038] (1) Preparation of bone targeting coupling molecules
[0039] ① Synthesis of the first intermediate
[0040] Dissolve dithiophenylmaleimide and N-methylmorpholine in ethyl acetate at a molar ratio of 1:1 to make the concentration of dithiophenylmaleimide 3mg / mL, and add in the dark Methyl chloroformate in an equimolar amount with dithiophenyl maleimide was stirred at room temperature for 1 hour. After the reaction, the crude product was separated by rotary evaporation. The crude product was dissolved in dichloromethane and washed with deionized water. Three times, then remove the water phase, keep the organic phase, add desiccant anhydrous MgSO to the organic phase 4 Remove the water in the organic phase, then filter to remove MgSO 4 Rotate to remove dichloromethane to obtain the first intermediate 3,4-dithiophenyl-2,5-dioxo-pyrrole-1-carboxylic acid ...
Embodiment 2
[0055] In this embodiment, a method for preparing bone-targeted salmon calcitonin is provided, and the steps are as follows:
[0056] (1) Preparation of bone targeting coupling molecules
[0057] ① Synthesis of the first intermediate
[0058] Dissolve dibromomaleimide and N-methylmorpholine in ethyl acetate at a molar ratio of 1:1.2 to make the concentration of dibromomaleimide 4mg / mL, and add methyl chloroformate in the dark , The amount of methyl chloroformate added is 1.2 times the equivalent molar amount of dibromomaleimide, and the reaction is stirred at room temperature for 2 hours. After the reaction is completed, the crude product is separated by rotary evaporation, and the crude product is dissolved in dichloromethane. Wash with deionized water three times, then remove the water phase, keep the organic phase, and add desiccant anhydrous MgSO to the organic phase 4 Remove the water in the organic phase, then filter to remove MgSO 4 Rotate to remove the dichloromethane to obt...
Embodiment 3
[0069] In this embodiment, a method for preparing bone-targeted salmon calcitonin is provided, and the steps are as follows:
[0070] (1) Preparation of bone targeting coupling molecules
[0071] ① Synthesis of the first intermediate
[0072] Dissolve dithiophenylmaleimide and N-methylmorpholine in ethyl acetate at a molar ratio of 1:1 so that the concentration of dithiophenylmaleimide is 2mg / mL. Add it in the dark Methyl chloroformate in an equimolar amount with dithiophenyl maleimide was stirred at room temperature for 1 hour. After the reaction, the crude product was separated by rotary evaporation. The crude product was dissolved in dichloromethane and washed with deionized water. Three times, then remove the water phase, keep the organic phase, add desiccant anhydrous MgSO to the organic phase 4 Remove the water in the organic phase, then filter to remove MgSO 4 Rotate to remove the dichloromethane to obtain the first intermediate 3,4-dithiophenyl-2,5-dioxo-pyrrole-1-carboxylic...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com