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Composition with xanthine oxidase inhibitory activity

A technique for inhibiting xanthine oxidase and activity, which is applied in the field of preparing xanthine oxidase inhibitors and compositions with xanthine oxidase inhibitory activity, can solve problems that have not been reported, achieve high safety, and increase raw material addition value, promote the effect of deep processing and utilization

Active Publication Date: 2018-10-09
ZHIREN WUHAN HEALTH IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the inhibition of xanthine oxidase by raspberry extract, and the combined use of the two extracts of Artemisia quinoa leaf for inhibition of xanthine oxidase

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Screening of Artemisia quinoa Leaf Extract for Inhibiting Xanthine Oxidase Effective Parts

[0016] (1) Preparation of each extraction part

[0017] Select fresh Artemisia quinoa leaves, dry them to constant weight, crush them through a 40-mesh sieve, then add 15 times the weight of 50% ethanol to reflux and extract 3 times, each extraction time is 1 hour, combine the extracts, and concentrate under reduced pressure to obtain an extract and placed in a separatory funnel.

[0018] Extract the extract with petroleum ether for 3 times, collect the extract, concentrate, and dry to obtain the petroleum ether part; the remaining extract is extracted with ethyl acetate for 3 times, collect the extract, concentrate, and dry to obtain ethyl acetate part; the remaining extract was extracted 3 times with n-butanol, and the extract was collected and concentrated and dried to obtain the n-butanol part; the remaining extract was dried, which was the water phase.

[0019] ...

Embodiment 2

[0024] Example 2 Preparation of Raspberry Extract and Detection of Its Inhibition of Xanthine Oxidase Activity

[0025] (1) Weigh the raspberry fruit, put it into a blender and pulverize and whip it to make it homogenate, adjust the pH of 70% ethanol solution to 2 with dilute hydrochloric acid as the extraction solvent, add 15 times to the raspberry homogenate weight of extraction solvent, ultrasonically extract for 1 hour, filter or centrifuge, collect the extract and concentrate under reduced pressure until there is no alcohol smell;

[0026] (2) Put the concentrated extract on the AB-8 type macroporous adsorption resin column, wash it with a hydrochloric acid aqueous solution with a pH of 2 at a speed of 0.25mL / s for 5h, and then wash it with a 70% ethanol solution with a pH of 2 at a rate of 2mL / min speed elution, collect 70% ethanol eluate and concentrate into a thick paste, and freeze-dry the thick paste to obtain.

[0027] Detect the inhibitory rate and IC of raspberr...

Embodiment 3

[0028] Preparation of Example 3 Composition and Detection of Inhibition of Xanthine Oxidase Activity

[0029] The Artemisia quinoa leaves ethyl acetate active fraction prepared in Example 1 and the raspberry extract prepared in Example 2 are respectively compatible in the proportions in Table 2, and then determined according to the method in Example 1 for each composition to xanthine Oxidase inhibition rate and IC 50 value.

[0030] See Table 2 for the results of the inhibition rate of each composition on xanthine oxidase at a concentration of 1 mg / mL.

[0031] Table 2 Inhibition rate of xanthine oxidase by different ratios of Artemisia quinoa leaf extract and raspberry extract composition

[0032] Raspberry Extract: Artemisia annua leaf ethyl acetate fraction

[0033] As can be seen from the above comparative results, the compositions of Artemisia annua Chenopodia leaf extracts and raspberry extracts in different proportions all have higher inhibitory rates to xan...

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PUM

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Abstract

The invention discloses novel application of artemisia selengensis leaf extract, raspberry extract and compositions thereof to preparation of xanthine oxidase inhibitors. The invention further provides a composition with the xanthine oxidase inhibitory activity. In the composition, a mass ratio of the artemisia selengensis leaf extract to raspberry extract is (1-10):1, preferably 8:1. According tothe application in the invention, a feasible novel pathway is provided for clinic treatment of hyperuricemia, the artemisia selengensis leaf extract and the raspberry extract serving as food sourceshave high safety, the application range of the artemisia selengensis and raspberry is widened, intensive processing and utilization are promoted, and additional values of the raw materials are increased.

Description

technical field [0001] The present invention relates to the new application of Artemisia quinoa leaf extract, raspberry extract and their compositions, especially the new application in the preparation of xanthine oxidase inhibitors, and the present invention also relates to a combination with xanthine oxidase inhibitory activity thing. Background technique [0002] In recent years, due to inappropriate lifestyles, the incidence of hyperuricemia has shown a younger trend, which seriously threatens people's health. Hyperuricemia is a disease caused by abnormal reabsorption and secretion of uric acid produced by purine metabolism in the kidneys and intestines due to abnormal genes and uric acid regulatory network. Hyperuricemia is the basis of gout, and is closely related to obesity, hyperlipidemia, hypertension, coronary heart disease, insulin resistance, and diabetic kidney damage, and is an important risk factor for cardiovascular diseases. Xanthine oxidase (XOD), as a ke...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61P19/06
CPCA61K36/28A61K36/73A61P19/06A61K2300/00
Inventor 徐晓云刘玲曹伟伟李慧潘思轶
Owner ZHIREN WUHAN HEALTH IND CO LTD
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