A method for inducing rapid healing of zhennan

A fast and sturdy technology, applied in the field of plant tissue culture in garden plant biotechnology, can solve the problems of slow growth, small reproduction coefficient and low survival rate.

Active Publication Date: 2021-09-14
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current propagation methods of Zhennan are mainly sowing and cutting, but the reproduction coefficient is small, the survival rate is not high, and the growth is slow, which is far from meeting the market demand, which seriously affects the development and utilization of Zhennan.

Method used

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  • A method for inducing rapid healing of zhennan
  • A method for inducing rapid healing of zhennan
  • A method for inducing rapid healing of zhennan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1), collection and cleaning of explants

[0034] The mother tree collected for explants is a one-year-old Zhennan seedling introduced from the Sichuan Academy of Forestry Sciences and planted in the Nongcui Garden of Anhui Agricultural University in March 2017. The new shoots germinated from the roots were selected, and then the branches were selected for growth. Robust, bright leaf color without spots, and young leaves free from diseases and insect pests are used as explants. Spray a little pure water + NAA0.1mg / L + grape seed extract proanthocyanidin 0.05g / L water-retaining agent on the surface of the young leaves for 5 minutes. Pick young leaves and bring them back to the laboratory to clean the explants. First, weigh 0.25g of washing powder and washing liquid and dissolve them in 250mL of water to obtain a cleaning solution. Then soak the explants in the cleaning solution for 10 minutes. Then carefully clean the surface of the explants, gently touch and wash the fr...

Embodiment 2

[0042] (1), collection and cleaning of explants

[0043] The mother tree collected for explants is the one-year-old Zhennan seedlings introduced from the Sichuan Academy of Forestry Sciences and planted in the Nongcui Garden of Anhui Agricultural University in March 2017. The newly grown branches from the top tips were selected, and then selected from the branches. The young leaves that grow vigorously, have bright leaf color without spots, and are free from diseases and insect pests are used as explants. Spray a little pure water + NAA2.5mg / L + grape seed extract proanthocyanidin 0.1g / L water retaining agent on the surface of the young leaves for 7 minutes. , take it back to the laboratory for explant cleaning, first weigh 0.25g of washing powder and washing liquid and dissolve it in 250mL of water to obtain a cleaning solution, then soak the explant in the cleaning solution for 15min, and then carefully wash the explant On the surface of the implant, gently touch and wash th...

Embodiment 3

[0051] (1), collection and cleaning of explants

[0052] The mother tree collected for explants is the one-year-old Zhennan seedlings introduced from the Sichuan Academy of Forestry Sciences and planted in the Nongcui Garden of Anhui Agricultural University in March 2017. The newly grown branches from the top tips were selected, and then selected from the branches. The young leaves that grow vigorously, have bright leaf color without spots, and are free from diseases and insect pests are used as explants. Spray a little pure water + NAA5.0mg / L + grape seed extract proanthocyanidin 0.2g / L water-retaining agent on the surface of the young leaves for 10 minutes. , take it back to the laboratory for explant cleaning, first weigh 0.25g of washing powder and washing liquid and dissolve it in 250mL of water to obtain a cleaning solution, then soak the explant in the cleaning solution for 20min, and then carefully wash the explant On the surface of the implant, gently touch and wash t...

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PUM

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Abstract

The invention relates to a method for inducing rapid healing of Phoebe zhennan. The young leaves of Phoebe zhennan are used as explants, and the rapid healing of Phoebe zhennan is completed through collection and cleaning of the explants, disinfection, cutting and inoculation, dark culture and light culture. The highest callus induction rate reaches 98.1%, and the degree of greening and callus growth is obvious, which will provide foundation and related technical support for callus induction of Zhennan, tissue culture and propagation of seedlings, acceleration of breeding of improved varieties, and meeting market demand in the future.

Description

technical field [0001] The invention relates to the field of plant tissue culture in garden plant biotechnology, in particular to a method for inducing rapid healing of zhennan. Background technique [0002] Zhennan, also known as Nanmu, Jinsinan or Xiaoyenan, is a slow-growing evergreen tree in the genus Phoebe of Lauraceae with a height of more than 40 meters. It grows slowly. The large trees of nearly a thousand years have no signs of aging. It is a national second-level key protection species. Phoebe zhennan bark is smooth, light gray-brown, because of its slow growth, the prismatic or cylindrical green branchlets are generally thinner, covered with gray-brown hairs, and the old branches have no hairs; the leaves are leathery, the young leaves are red, the old leaves are green, the apex is acuminate, The base is wedge-shaped, the upper surface of the leaf is bright and glabrous, the lower part is densely pubescent, the leaf veins are longer, the ventral midrib is sunken...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 刘桂华余云云许早时邓波赵惠岳祥华雷刚詹文勇周立琪
Owner ANHUI AGRICULTURAL UNIVERSITY
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