Collector of flow cytometer

Abstract

The invention discloses a collector of a flow cytometer. The collector comprises a separation bin comprising at least three compartments, wherein a liquid guide hopper is integrally prepared at the bottom of each compartment of the separation bin; clamp plates are fixed on the two outer sides of the separation bin; a liquid collection frame is fixed at the bottoms of the clamp plates; a flange plate is fixed at the bottom of the inner side of the liquid collection frame; screw rods are rotationally connected to the flange plate; stress cups are fixed at the tops of the screw rods; collection cups are arranged on the inner sides of the stress cups; tops of the collection cups just face the liquid guide hoppers. The collector of the flow cytometer is provided with the square compartments ofthe separation bin, drop liquids from a deflecting plate can be received totally, pollution to ambient environment is avoided, and the drop liquids collected by the collection cups can be guided out by the flow guide hoppers for analysis experiments.

Description

technical field [0001] The invention relates to a flow cytometer component, in particular to a flow cytometer collector, and belongs to the technical field of flow cytometer components. Background technique [0002] The sorting of cells by flow cytometry is achieved by forming charged droplets containing cells by the flow beam; the nozzle of the flow cell of the flow cytometer with sorting function is equipped with an ultrasonic vibration pressing crystal sheet, and the vibration The device can break the liquid beam ejected from the nozzle into thousands of small droplets, and the flowing cells are dispersed in these small water droplets at the same time. At this time, an electric pulse signal is given to the stream, so that all the small water droplets are charged. When the cell fluid with different charges flows through a pair of deflection plates with positive and negative constant electrostatic electric fields of several thousand volts, the charged water droplets will b...

AI Technical Summary

Problems solved by technology

When the cell fluid with different charges flows through a pair of deflection plates with positive and negative constant electrostatic electric fields of several thousand volts, the charged water droplets will be deflected according to their own charge properties and fall into their respective collection containers. The uncharged water droplets enter the waste liquid container in the middle, thereby realizing the sorting of cells; the existing collector for flow cytometry is composed of test tubes and flasks, which are respectively caught in different directions. Separated droplets have different offsets due to the different charges carried by the droplets. However, when the existing collectors collect droplets with different charges, there are droplets waste, and it is easy to shift to the outside of the collection container, causing pollution to the workbench; for this reason, Chinese patent application number: 201720073588.2 discloses a collector for flow cytometry for medical testing, including a waste liquid beaker, a Grade test tube, secondary test tube, test tube slope, and beaker slope, the fixed plate is provided with through holes matching the waste liquid beaker, primary test tube, and secondary test tube, and the waste liquid beaker, primary test tube and secondary The top of the test tube is fixed in the through hole, and the tops of the waste liquid beaker, the first-level test tube and the second-level test tube are provided with a truncated beaker on the fixed plate, and the outer side of the waste liquid beaker is provided with a beaker positioning ring. Test tube positioning rings are arranged on the outside of the first-level test tube and the second-level test tube, and between the two adjacent test tube positioning rings, between the beaker positioning ring and the test tube positioning ring are connected by connecting rods

The collector of the flow cytometer for medical inspection can realize the classification and collection of weakly charged droplets and strongly charged droplets by using the first-level test tube and the second-level test tube, which is convenient for different experiments in the later stage, and can effectively reduce The waste of droplets; but it can only complete the classification and collection of droplets, but it still causes pollution to the outer periphery of the collector

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