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Adenosine and andrographolide compound drug composition and application thereof

A technology of andrographolide and adenflowerin, which is applied in the field of natural medicines, can solve problems such as unreported effects, achieve good prospects for clinical drug development, strong anti-proliferation activity, and reduce the effects of toxic and side effects

Active Publication Date: 2018-11-02
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The synergistic effect of ADE and its combination with ADH on lymphoma has not been reported

Method used

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  • Adenosine and andrographolide compound drug composition and application thereof
  • Adenosine and andrographolide compound drug composition and application thereof
  • Adenosine and andrographolide compound drug composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: adopt Daudi cell, Raji cell and BC-3 cell to screen and optimize adenflowerin (ADE) and andrographolide (ADH) compound pharmaceutical composition

[0022] Take the cells in the logarithmic growth phase and inoculate 3×10 4 Cells / well were placed on a 96-well plate. After 6 hours of growth, the supernatant was discarded by centrifugation, and then administered according to the following groups: Tumor cells were divided into no drug group and drug-added group, and the drug-added group was divided into ADH and ADE single drugs. group, ADH and ADE combined drug group with different molar ratios, set 4-6 duplicate wells in each group, culture for 24 hours, discard the supernatant, add 100 μl of MTT (tetrazolium salt) serum-free culture medium containing 0.5 mg / ml for culture After 4 hours, add 100 μl of DMSO (dimethyl sulfoxide), place on a micro-oscillator to vibrate for 10 minutes, and then place on a microplate reader to detect the OD value at 570 nm. Resul...

Embodiment 2

[0028] Embodiment 2: Analysis of the combined administration effect of ADE and ADH

[0029] Based on the Median-effect Principle (moderate effect principle or Chou-Talalay combined index method), the dose-response curve and the combined index curve (fa-C curve) under different effects were drawn using CombiDrug statistical software. The relationship between the effect and the combination index quantitatively evaluates whether there is a synergistic, antagonistic or additive relationship between the two drugs. Specific steps are as follows:

[0030] The drug effect is the inhibition rate (fa)=1-(the average OD570 value of the test group / the average OD570 of the tumor cell blank control group) according to the intermediate effect equation fa / fu=(D / Dm) m , take the logarithm logfa / fu=mlogD–mlogDm on both sides, set a=-mlogDm, b=m, x=logD, y=logfa / fu, and substitute into the intermediate effect equation to get y=bx–a; where fa is the drug effect , fu=1–fa, D is the drug concentr...

Embodiment 3

[0036] Example 3: Safety of ADE and ADH on peripheral blood mononuclear lymphocytes

[0037]The safety of ADH and ADE on PBMC cells was evaluated after isolation and culture of mononuclear lymphocytes from healthy volunteers ( figure 1 ): ① draw fresh blood from healthy volunteers into a heparin anticoagulant tube (sterile); then resuspend the cells (sterile) with an equal volume of PBS (or serum-free D-Hank buffer); ② put the suspended cells Add the pre-coated human lymphocyte separation medium (preheated at 37°C), the volume ratio of lymphatic separation medium to cell suspension is not less than 1:1; ③ 500×g (or 2000rpm) horizontal centrifuge for 20-30min, room temperature (20-30°C) at slow speed; ④ Discard the upper layer of plasma, carefully suck out the middle white mist layer and add it to 5ml (or 1-2 times the volume) of PBS (or serum-free cell culture buffer), 200×g or 1000rpm Centrifuge for 10 minutes at room temperature (20-30°C) at a slow speed, discard the supern...

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Abstract

The invention belongs to the field of natural drugs and discloses an adenosine and andrographolide compound drug composition and application thereof. An active ingredient of the compound drug composition consists of adenosine and andrographolide with a molar ratio of 1:1-1:20. The combination of the two drugs has a strong application to the synergistic treatment of lymphoma.

Description

technical field [0001] The invention belongs to the field of natural medicines, in particular to a compound pharmaceutical composition of adenflowerin and andrographolide and its application. Background technique [0002] Cancer is a chronic disease that seriously endangers human health, and has become the second largest killer after cardiovascular disease. Therefore, it is of great significance to find safe, effective, and low-toxic antitumor drugs and study their mechanism of action. [0003] Adenanthin (Adenthin, ADE), a small molecule diterpenoid compound extracted from a plant called Adenanthin in Yunnan, my country. Adenanthin is a naturally occurring compound in a plant with a high safety. Recent studies have found that ADE can induce leukemia APL differentiation and kill liver cancer cells (Nat Chem Biol.2012Apr 8; 8(5):486-93. Cell Death Dis.2014Sep 4; 5:e1400.), has the ability to develop into new tumors drug potential. [0004] Andrographolide (ADH), a diterpene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/365A61K31/22A61P35/00
CPCA61K31/22A61K31/365A61P35/00A61K2300/00
Inventor 刘连陈建萍彭咏波刘腾李雄崔亮刘武昆谢国健
Owner FOSHAN UNIVERSITY
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