Preparation method of marine filariasis vaccine of redfin puffer

A technology for red fin puffer and cercarial filariasis, which is applied in the field of vaccine preparation, can solve the problem that there is no vaccine for red fin puffer marine cercariasis, the treatment method of Chinese herbal medicine is ineffective, and the marine tail silk cannot be completely killed Insects and other problems, to achieve the effect of defense against parasites and infections, improve immunity, and reduce economic losses

Active Publication Date: 2021-05-11
DALIAN OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physiotherapy methods such as fresh water baths cannot completely kill marine C.
So far, there is no report on the preparation of a vaccine against marine filariasis in redfin puffer

Method used

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  • Preparation method of marine filariasis vaccine of redfin puffer
  • Preparation method of marine filariasis vaccine of redfin puffer
  • Preparation method of marine filariasis vaccine of redfin puffer

Examples

Experimental program
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Embodiment

[0018] a. The worm body was isolated from the festered tissue of the redfin fugu body surface in a farm, and was identified as the larvae of the marine filariasis ( Uronema marinum ). Inoculate the isolated parasites into broth medium, and carry out in vitro acclimatization and cultivation for 15 days to obtain the liquid of marine cercariasis in vitro acclimatization culture. The broth medium is boiled with 5 g of homogenized fish meat per 100 ml of sterilized seawater 10min, then filter the filtrate with 200 mesh gauze;

[0019] b. Inoculate the cultured marine C. filariae in vitro into the broth medium, the volume ratio of the inoculum to the broth medium is 1:100, and cultivate at 20°C for 120 hours to obtain C. marine C. liquid;

[0020] c. Draw 1ml of marine C. filariae liquid in the growth phase into a sterilized 1.5ml centrifuge tube, centrifuge at 2000rpm for 10 minutes, discard the supernatant and add 1ml L-15 complete medium to the centrifuge tube, the L- The 15...

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Abstract

The invention discloses a method for preparing a marine filariasis vaccine of redfin puffer. A single marine filariasis is picked and inoculated in L-15 complete medium for purification and cultivation, and then enlarged and cultivated, and 100 μl / L of Foreria is added. Marin killed the larvae, centrifuged at 4000rpm in a low-speed centrifuge for 10 minutes to collect the precipitate, added L‑15 medium to prepare an inactivated larvae liquid with a density of 2 million / ml, collected in a 1.5ml centrifuge tube, and stored at ‑80°C. Before immunization, thaw the dead larvae on ice, crush them with electromagnetic waves, mix them with the same amount of Freund's adjuvant or Freund's incomplete adjuvant, and shake the mixture until it is in the form of emulsion, which is to prepare the marine tail silk of puffer puffer Insect vaccines. Intraperitoneal injection can improve the immunity of pufferfish against marine filariasis.

Description

technical field [0001] The invention relates to a preparation method of a vaccine, in particular to a preparation method of a vaccine for preventing marine filariasis of redfin puffer. Background technique [0002] The marine filariasis belongs to the shield ciliates, which is a small facultative parasitic ciliate. It parasitizes the red-finned puffer and can cause the marine filariasis of the red-finned puffer. The disease is highly contagious and has a wide range of transmission. It can cause increased mucus on the body surface, blackened body color, white turbidity, ulceration or peeling of the back, redness and swelling of the head, swelling, congestion, water accumulation inside the gill cover, discoloration or erosion of the gills, and abdominal pain. Swelling, local swelling, hemorrhage or ulceration, symptoms such as fin hemorrhage and ulceration cause a large amount of economic losses to the redfin pufferfish aquaculture industry. Therefore, the prevention of marin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/002A61K39/39A61P33/02A61P37/04
CPCA61K39/002A61K39/39A61K2039/521A61K2039/552A61P33/02A61P37/04
Inventor 黎睿君高延奇叶仕根张涛
Owner DALIAN OCEAN UNIV
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