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Cordyceps militaris culture method

A cultivation method, technology of Cordyceps militaris, applied in the field of cultivation of Cordyceps militaris, can solve problems such as transformation

Active Publication Date: 2018-11-16
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current culture method of Cordyceps militaris, the Cr in the inorganic state has not been cultured by enriching chromium. 3+ Reports on Transition Organic State

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0036] A method for cultivating Cordyceps militaris, said method comprising the steps of:

[0037] (1) VB the Cordyceps militaris strains preserved at 0-4°C 1 Incline culture:

[0038] A, preparation of Cordyceps militaris culture medium: 200 parts by weight of potatoes are cut into small pieces of 0.8-1.2cm, boiled in boiling water for 20 minutes, then filtered, juice is added, and 20 parts of glucose and magnesium sulfate are added 0.5 parts, 1 part potassium dihydrogen phosphate, vitamin B 1 0.01 part, 20 parts of agar, after melting, set the volume to 1000mL, natural pH value;

[0039] B, subpackage of Cordyceps militaris culture medium, sterilization:

[0040] Divide the prepared culture medium into 18mm×180mm test tubes, seal, and sterilize at 121°C for 20 minutes;

[0041] C, cooling, inoculation:

[0042] After sterilization, take out the culture medium of Cordyceps militaris, cool naturally to 30°C, and insert the Cordyceps militaris strain under aseptic conditio...

Embodiment 2

[0055] A method for cultivating Cordyceps militaris, said method comprising the steps of:

[0056] (1) VB the Cordyceps militaris strains preserved at 0-4°C 1 Incline culture:

[0057] A, preparation of Cordyceps militaris culture medium: 200 parts by weight of potatoes are cut into small pieces of 0.8-1.2cm, boiled in boiling water for 20 minutes, then filtered, juice is added, and 20 parts of glucose and magnesium sulfate are added 0.5 parts, 1 part potassium dihydrogen phosphate, vitamin B 1 0.01 part, 20 parts of agar, after melting, set the volume to 1000mL, natural pH value;

[0058] B, subpackage of Cordyceps militaris culture medium, sterilization:

[0059] Divide the prepared culture medium into 18mm×180mm test tubes, seal, and sterilize at 121°C for 20 minutes;

[0060] C, cooling, inoculation:

[0061] After the sterilization, take out the culture medium of Cordyceps militaris, cool naturally to 25°C, and insert the Cordyceps militaris strain under aseptic cond...

Embodiment 3

[0074] A method for cultivating Cordyceps militaris, said method comprising the steps of:

[0075] (1) VB the Cordyceps militaris strains preserved at 0-4°C 1 Incline culture:

[0076] A, preparation of Cordyceps militaris culture medium: 200 parts by weight of potatoes are cut into small pieces of 0.8-1.2cm, boiled in boiling water for 20 minutes, then filtered, juice is added, and 20 parts of glucose and magnesium sulfate are added 0.5 parts, 1 part potassium dihydrogen phosphate, vitamin B 1 0.01 part, 20 parts of agar, after melting, set the volume to 1000mL, natural pH value;

[0077] B, subpackage of Cordyceps militaris culture medium, sterilization:

[0078] Divide the prepared culture medium into 18mm×180mm test tubes, seal, and sterilize at 121°C for 20 minutes;

[0079] C, cooling, inoculation:

[0080] After sterilization, take out the culture medium of Cordyceps militaris, cool naturally to 20°C, and insert the Cordyceps militaris strain under aseptic conditio...

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Abstract

The invention discloses a cordyceps militaris culture method. According to the method, a proper culture medium and a proper culture condition are selected, and Cr<3+> of an inorganic state is converted into organic chromium through the enrichment effect of mycelia to Cr<3+>, so that the inorganic Cr<3+> is effectively prevented from being naturally oxidized to Cr<6+>, and the damage to human bodies caused by direct absorption of the metal Cr<3+> by the human bodies is reduced. The study also finds that the chromium in the cordyceps militaris also promotes the conversion of polysaccharide in the mycelia, and meanwhile, the pancreatic function of diabetic patients can be improved through the combined action of the organic chromium and cordyceps polysaccharide, so that the use of artificial insulin is reduced.

Description

technical field [0001] The invention relates to the technical field of strain cultivation, in particular to a method for cultivating Cordyceps militaris. Background technique [0002] Cordyceps militaris L.Link, also known as Cordyceps militaris, Cordyceps militaris, Militaris, etc., according to the latest classification system, Cordyceps militaris belongs to Ascomycota, Hypocreales, Cordycipitaceae, Cordyceps The genus Cordyceps (Sung et al., 2007). Cordyceps militaris has similar active ingredients and pharmacological effects as Cordyceps sinensis (Kim and Yun, 2005; Li et al., 2006; Wang Jianfang and Yang Chunqing, 2005), and the fermentation products of Cordyceps sinensis have antibacterial, antioxidant, anti-inflammatory, anti-tumor, immune enhance various biological activities. Among them, Cordyceps polysaccharide has a significant hypoglycemic effect on a variety of diabetic model animals, and its mechanism may be related to inhibiting hepatic glucose output, promo...

Claims

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Application Information

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IPC IPC(8): A01G18/00A01G18/20
CPCA01G18/00A01G18/20
Inventor 王建瑞刘宇刘悦李娟刘震彭炜航
Owner LUDONG UNIVERSITY
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