CAR-T cellular automation preparation system based on micro-fluidic chip

A microfluidic chip and preparation system technology, applied in biochemical equipment and methods, methods for supporting/immobilizing microorganisms, and culturing devices for tissue cells/viruses, etc., can solve constraints on the development and popularization of CAR-T cell therapy technology. and other problems to achieve the effect of reducing human participation, ensuring miniaturization, and avoiding pollution

Active Publication Date: 2018-11-20
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research direction of the automatic preparation system of CAR-T cells has been neglected by scholars, and it is still in a blank period, which restricts the development and application of CAR-T cell therapy technology to a certain extent.

Method used

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  • CAR-T cellular automation preparation system based on micro-fluidic chip
  • CAR-T cellular automation preparation system based on micro-fluidic chip
  • CAR-T cellular automation preparation system based on micro-fluidic chip

Examples

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Embodiment 1

[0064] Such as figure 1 The CAR-T cell automatic preparation system shown includes a microfluidic chip 1, and the microfluidic chip is provided with a sampling port. In this embodiment, it is connected to a syringe pump sampling system 2, and the original blood sample passes through The pushing of the push rod enters the microfluidic chip from the front outlet of the syringe, and the microfluidic chip also includes a product outlet 101 and a non-product outlet 102; it also includes a culture bag 3, and the culture bag includes an inlet The liquid port 301 and the liquid outlet port 302, the product outlet port 101 of the microfluidic chip communicates with the liquid inlet port 301 of the culture bag through the first pipeline 401, and the first pipeline is also provided with a sample port, and the sample port Connect three sampling pipelines respectively, which are respectively the non-product outlets of the microfluidic chip described in the lentivirus suspension sampling pi...

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Abstract

The invention relates to the field of cell culture and in particular relates to a CAR-T cellular automation preparation system based on a micro-fluidic chip. The CAR-T cellular automation preparationsystem comprises a micro-fluidic chip, a culture bag and a product bag, wherein the components are connected through pipelines. With the adoption of the closed loop automation scheme, production and preparation of cell therapy products can be performed; firstly, the requirements on technical personnel can be reduced, and uncertain risk brought by artificial participation in the product preparationprocess is avoided; secondly, the corresponding manual cost and GMP clean room cost can be obviously reduced, and the whole cell therapy cost is further reduced.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a culture of CAR-T cells, and in particular to an automatic preparation system for CAR-T cells based on a microfluidic chip. Background technique [0002] Conquering cancer has always been the dream of biomedical workers. With the rapid development of related disciplines such as oncology, immunology and molecular biology, the emergence of tumor immunotherapy has made it possible to achieve this goal. The mechanism of tumor immunotherapy starts from the origin of the body's immunity to cancer monitoring, and eliminates or controls tumor cells by re-stimulating or mobilizing the body's immune system. At present, chimeric antigen receptor T cell (CAR-T) immunotherapy has the most eye-catching performance in the existing technology. In this method, the T cells in the patient's peripheral blood can be expressed in vitro through genetic engineering to specifically recognize tumor cells, a...

Claims

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Application Information

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IPC IPC(8): C12M3/02C12M1/34C12M1/12C12M1/00
CPCC12M23/14C12M23/28C12M27/16C12M33/14C12M35/08C12M41/36C12M47/04
Inventor 肖忠党郭士成孙博叶正
Owner SOUTHEAST UNIV
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