Pretreatment kit and method for bacterial plasmid extraction
A bacterial plasmid and kit technology, which is applied in the field of pretreatment kits for bacterial plasmid extraction, can solve the problems of difficult plasmid extraction, high cross-linking degree, and high cost, and overcome the inability to effectively extract Gram-positive bacterial plasmids, Broad market application prospects, excellent extraction effect
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Embodiment 1
[0029] Embodiment 1, the pretreatment kit and method of bacterial plasmid extraction of the present invention
[0030] 1. Kit composition
[0031] A component: 100mg / ml lysozyme
[0032] B component: 5% (refers to 5g / 100ml) glucose solution
[0033] 2. How to use
[0034] Take the bacteria, add 100 mg / ml lysozyme, and incubate at 37°C for 10 minutes. After lysozyme treatment, centrifuge the suspension at 11,000×g for 1 minute at room temperature; Gently suspend in 5% glucose solution, centrifuge at 11,000×g for 1 minute, and discard the supernatant.
Embodiment 2
[0035] Embodiment 2, the pretreatment kit and method of bacterial plasmid extraction of the present invention
[0036] 1. Kit composition
[0037] Component A: 120mg / ml lysozyme
[0038] Component B: 4% glucose solution
[0039] 2. How to use
[0040] Take the bacteria, add 150 mg / ml lysozyme, and incubate at 37°C for 10 minutes. After lysozyme treatment, centrifuge the suspension at 11,000×g for 1 minute at room temperature; Gently suspend in 5% glucose solution, centrifuge at 11,000×g for 1 minute, and discard the supernatant.
Embodiment 3
[0041] Embodiment 3, the pretreatment kit and method of bacterial plasmid extraction of the present invention
[0042] 1. Kit composition
[0043] A component: 160mg / ml lysozyme
[0044] Component B: 5% glucose solution
[0045] 2. How to use
[0046] Take the bacteria, add 200mg / ml lysozyme, and incubate at 37°C for 10 minutes. After lysozyme treatment, centrifuge the suspension at 11,000×g for 1 minute at room temperature; Gently suspend in 5% glucose solution, centrifuge at 11,000×g for 1 minute, and discard the supernatant.
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