A paternity testing method based on fetal cell-free DNA in the peripheral blood of pregnant women
A technology for maternal peripheral blood and paternity testing, which is applied in the field of paternity testing based on fetal cell-free DNA in maternal peripheral blood, can solve problems such as insufficient sequencing depth, lack of statistical models for genetic evidence value indicators, and misjudgment, so as to avoid medical risks. , high identification efficiency, high analysis efficiency
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[0122] It involves undetermined male 1, pregnant woman M and undetermined male 2. Carry out paternity test according to the method provided by the present invention, the steps are as follows:
[0123] Step 1, DNA extraction:
[0124] A commercially available DNA extraction kit was used to extract the genomic DNA from the exfoliated cells of the throat swab of pregnant woman M, and to extract the cfDNA from the peripheral blood of pregnant woman M; at the same time, the genomic DNA from the peripheral blood of unidentified male 1 and unidentified male 2 was extracted.
[0125] Step 2, next-generation sequencing:
[0126] For the genomic DNA of M, A, and F, the library was built according to the resequencing of genomic DNA, and the average sequencing depth was not less than 10 times; the peripheral blood cfDNA of M was captured and built using the Agilent whole exome capture sequencing kit. Library sequencing, the average sequencing depth is not less than 200 times. Among...
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