Method for rapidly screening ERp57 inhibitors by high throughput
An inhibitor, high-throughput technology, applied in the field of biomedicine, can solve the problems of high cost, low screening efficiency, environmental pollution, etc., and achieve the effect of low cost and sample saving.
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[0020] The following examples further illustrate the content of the present invention, but should not be construed as limiting the present invention. Any modifications or substitutions made to the methods, steps or conditions of the present invention without departing from the essence of the present invention fall within the scope of the present invention. Unless otherwise specified, the technical means used in the embodiments are conventional means well known to those skilled in the art.
[0021] All reagents used in the examples are commercially available unless otherwise specified.
[0022] The sample to be screened can be a traditional Chinese medicine extract or a mixture of multiple compounds. In this patent, the extract of the traditional Chinese medicine Salvia miltiorrhiza is taken as an example.
[0023] 1. Preparation of ERp57 protein with His tag:
[0024] Add 10 ml of binding buffer to suspend 2 g wet weight of E. coli BL 21 strain (transformed with the constru...
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