A method for identifying hibiscus bark in a compound traditional Chinese medicine

Abstract

The invention discloses a method for identifying cortex pseudolaricis in a traditional Chinese medicinal compound. The method comprises the following steps: (1) adding a sample onto a polyamide column, eluting with a mixed solution, eluting with an organic solvent A, collecting the eluting liquid, drying by distillation, dissolving residues with the organic solvent to serve as a test-sample solution; (2) treating a cortex pseudolaricis control medicinal material with an organic solvent B, drying the filtrate by distillation, dissolving the residues with the organic solvent B to serve as a control medicinal material solution; and (3) taking 2-6mu L of the test sample solution and 2-6mu L of the control medicinal material solution, respectively applying the solutions to the same silica gel chromatographic sheet, taking a mixed solution of cyclohexane-ethyl acetate as a developing solvent, developing, taking out, drying in the air, and inspecting under a 365nm ultraviolet lamp. The identification method can be used for effectively removing impurities in a sample for test, the main ingredient loss is relatively small, and the chromatogram of the test-sample solution has clear and easily-distinguished spots, application of benzene and other reagent with relatively high toxicity can be avoided. The method has the advantages of high specificity, good repeatability, high sensitivity, environment friendliness and the like.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine detection, and in particular relates to a method for identifying hibiscus bark in a compound traditional Chinese medicine. Background technique [0002] Compound soil hibiscus bark tincture is a compound preparation of traditional Chinese medicine, which is recorded in the 17th volume of traditional Chinese medicine preparations in the "Drug Standards of the Ministry of Health of the People's Republic of China". The standard number is: WS3-B-3269-98. For toe itching, skin itching, general skin ringworm. The prescription consists of 42g of hibiscus bark, 125g of benzoic acid, and 62.5g of salicylic acid. The preparation method is as follows: take the above three flavors, take hibiscus bark, add appropriate amount of ethanol, heat and reflux for 5 hours, and filter; mix benzoic acid and salicylic acid Dissolve with ethanol, combine with hibiscus bark filtrate, add ethanol to ma...

AI Technical Summary

Problems solved by technology

[0004] The TLC identification method in the quality standard of compound soil hibiscus bark tincture was tested on the compound soil hibiscus bark tincture. The test results showed that there were no spots or unclear spots on the corresponding positions of the chromatogram of the test product and the reference medicinal material in the same batch, which affected the results. Judgment or misjudgment that the product quality is unqualified

Trace it to its cause: 1. the chemical composition in the test solution is complex without purification treatment, directly take the test solution to spot the sample, which interferes with the detected components; Large, the spots are easy to overlap to the front of the solvent, and the effective separation cannot be achieved, which affects the judgment of the results; The ingredients will all show the same color, and it is impossible to distinguish different types of organic chemical ingredients. The specificity is poor, which affects the judgment of the identification result.

However, in the identification method of Sun Hongying et al., the pretreatment steps of the test solution are cumbersome and require the use of toxic reagent benzene, which is very unfavorable for the health of the experimenter and the protection of the environment.

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