Megalurothrips usitatus aggregation pheromone and application thereof

A technology for gathering pheromones and thrips beanus, which is applied in the fields of application, chemicals for biological control, animal repellents, etc., can solve the problems that there are no reports on the development and application technology of thrips beanus aggregated pheromones, etc. Achieve the effects of low product price, low insect population density and cost reduction

Active Publication Date: 2018-11-30
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports on the aggregation pheromones of Thrips beanus and its application technology at home and abroad. Han Yun of South China Agricultural University studied the attraction effect of plant volatiles on Thrips beanus in his master's thesis, but there is no relevant information about Thrips beanus. Development and Application Technology Report of Thrips Aggregation Pheromone

Method used

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  • Megalurothrips usitatus aggregation pheromone and application thereof
  • Megalurothrips usitatus aggregation pheromone and application thereof
  • Megalurothrips usitatus aggregation pheromone and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1, identification of the active ingredient of the aggregation pheromone of Thrips beanus and preparation of the lure

[0051] (1) Separation and purification of pheromones: two methods were used to separate and purify aggregated pheromones of Thrips japonica.

[0052] 1) Use the headspace purge and trap method. The flowing air is generated by a vacuum pump, and the collection device is a glass tube filled with activated carbon, a gas washing bottle, a glass tube with an odor source, odor adsorbent Tenax, and a vacuum pump. After collecting the odor of Thrips fabae for 48 hours, the odor adsorbent was eluted with chromatographically pure n-hexane:ethyl acetate=95:5, passed through a 0.5 μm filter membrane and stored in a -20°C refrigerator for subsequent detection.

[0053] 2) Using solid-phase microextraction. Put pheromone-producing thrips into a 40 mL headspace glass vial, absorb it with an extraction head (Supelco, 57236-U) for 4 hours, and then inject gas ...

Embodiment 2

[0061] The luring effect of thrips adults of different sexes on the same species of thrips was measured by Y-shaped olfactometer. The test device consists of a gas generating device, an odor arm, and a Y-shaped glass tube, and the different parts are connected by Teflon tubes and Teflon tapes. The air generated by the atmospheric sampler passes through the activated carbon and the scrubber in sequence, then enters the two odor arms respectively, mixes with the volatile substances generated in the odor arms, and finally enters the selection arm that enters the Y-shaped tube respectively, and finally merges in the adaptation arm . The gas flow rate is 30mL / min, and the ambient temperature is 29±1°C. One of the odor arms was put into an odor source, and the odor source was a mixture of male and female worms, male worms or female worms that had just molted into adults, and the other odor arm was not put into an odor source as a blank control. In the adaptation arm of the Y-shape...

Embodiment 3

[0065] The attraction effect of different concentrations of pheromones on Thrips fabaceae was tested by Y-shaped olfactometer. The standard samples of geranyl acetate and farnesyl acetate were mixed according to the ratio of 3:1, and then the standard samples were configured into different concentrations with paraffin oil, and the specific mass percentage concentrations were 10%, 30%, 50%, and 70%. The concentrations of pheromones that attract thrips were measured by Y-shaped olfactometer. The method is the same as step (1) in the embodiment of the present invention, a filter paper of 1 × 4 cm is placed in one odor arm, and 10 μ L of different concentrations of pheromones are added to the filter paper; the same filter paper is placed in the other odor arm as a blank control, The result is as Figure 5 shown. Depend on Figure 5 It shows that the aggregated pheromones with the concentration of 30%-70% all have an attractive effect on Thrips grandis.

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Abstract

The present invention discloses a megalurothrips usitatus aggregation pheromone and an application thereof. The main active components of the megalurothrips usitatus aggregation pheromone are geranylacetate and farnesyl acetate. The megalurothrips usitatus aggregation pheromone is applied in preparing a product for forecasting and predicting and/or controlling megalurothrips usitatus. An attraction core used for attraction and aggregation of the megalurothrips usitatus comprises the megalurothrips usitatus aggregation pheromone and a sustained release carrier. The megalurothrips usitatus aggregation pheromone has characteristics of being micro in amount, high in efficiency, specific, and non-toxic and non-polluting to the environment.

Description

technical field [0001] The invention relates to an aggregation pheromone of Thrips beanus and its application, and belongs to the field of plant protection preparations. Background technique [0002] Thrips beanus is a pest that seriously damages cowpea in Hainan Province and other areas. In 2013, it was first reported in 2013 that the main species of thrips that mainly damage Hainan cowpea are Thrips grandis, Thrips flower, and Thrips palmi. Among them, the number of thrips beanus accounted for 97.3% of the total, and it was the dominant population of thrips beanus. The bean thrips Megalurothrips usitatus (Bagnall) belongs to the order Thysanoptera, the suborder Terebrantia, and the thrips family Thripidae. The host has reported damage to 17 plants so far, and prefers to damage leguminous and solanaceous crops. There are two main ways of damage: one is to cause direct damage through feeding and spawning behavior; the other is to spread by carrying pollen Viruses cause in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N37/02A01P19/00A01M1/02
CPCA01M1/02A01N37/02
Inventor 石旺鹏刘平平俸明月张柳秦紫芳张雨馨
Owner CHINA AGRI UNIV
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