Gastric carcinoma diagnosis kit combining RhoA signal path monitoring with microRNAs (micro Ribonucleic Acids) and application thereof

A diagnostic kit and signaling pathway technology, applied in the field of gastric cancer diagnostic kits, can solve the problems of low early diagnosis rate of gastric cancer, achieve high sensitivity and specificity, good application prospects, and expand the scope of application

Inactive Publication Date: 2018-12-11
深圳瑞科生物科技有限公司
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Problems solved by technology

The vast majority of gastric cancer is adenocarcinoma. There are no obvious symptoms in the early stage, or non-specific symptoms such as epigastric discomfort and belching, which are often similar to the symptoms of chronic gastric diseases such as gastritis and gastric ulcer, and are easily ignored. Therefore, the early diagnosis of gastric cancer in my country is currently rate is still low

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  • Gastric carcinoma diagnosis kit combining RhoA signal path monitoring with microRNAs (micro Ribonucleic Acids) and application thereof
  • Gastric carcinoma diagnosis kit combining RhoA signal path monitoring with microRNAs (micro Ribonucleic Acids) and application thereof
  • Gastric carcinoma diagnosis kit combining RhoA signal path monitoring with microRNAs (micro Ribonucleic Acids) and application thereof

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Embodiment Construction

[0028] A gastric cancer diagnostic kit for monitoring RhoA signaling pathway and microRNAs provided by the present invention and its application will be further described below in conjunction with specific examples. The embodiments described below are exemplary only for explaining the present invention and should not be construed as limiting the present invention.

[0029] The experimental methods in the following examples are conventional methods unless otherwise specified. The experimental materials used in the following examples were purchased from the market unless otherwise specified.

[0030] This embodiment provides a gastric cancer diagnostic kit for monitoring RhoA signaling pathway and microRNAs, which constitute the core of the kit, that is, detection of RhoA, miR-17 specific primers and fluorescent probes.

[0031] Specifically, the reagents in this kit include: RhoA detection primers: RhoAF, RhoAR, fluorescent probe RhoAP; miR-17 detection primers: miR-17F, miR-1...

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Abstract

The invention provides a gastric carcinoma diagnosis kit combining RhoA signal path monitoring with microRNAs (micro Ribonucleic Acids). The gastric carcinoma diagnosis kit comprises an RhoA detectionprimer, a fluorescent probe RhoAP, an miR-17 detection primer, a fluorescent probe miR-17P and an internal reference quality control sequence which takes human-derived beta-actin as a target gene. The gastric carcinoma diagnosis kit provided by the invention is used for detecting gastric carcinoma and has higher sensitivity and higher specificity, false positive of an immunological detection method is avoided, and a simple and fast detection means is provided for clinic individualized treatment.

Description

technical field [0001] The invention relates to the technical field of gene detection of diseases, in particular to a gastric cancer diagnostic kit for monitoring RhoA signaling pathway and microRNAs and its application. Background technique [0002] Gastric cancer (gastric carcinoma) is a malignant tumor originating from the gastric mucosal epithelium. It ranks first in the incidence of various malignant tumors in my country. The incidence of gastric cancer has obvious regional differences. The incidence of gastric cancer in the northwest and eastern coastal areas of my country is higher than that in the south. area is significantly higher. The age of onset is more than 50 years old, and the ratio of male to female incidence is 2:1. Due to changes in diet structure, increased work pressure, and Helicobacter pylori infection, gastric cancer tends to be younger. Gastric cancer can occur in any part of the stomach, and more than half of them occur in the gastric antrum. The g...

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/166C12Q2600/178
Inventor 翟雷垒廖兴华郭伟李馥正项园
Owner 深圳瑞科生物科技有限公司
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