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Latrodectus tredecimguttatus egg granule protein with anti-breast cancer activity, and preparation method and application thereof

An anti-breast cancer and spider spider technology, applied in the direction of peptide/protein components, medical preparations containing active ingredients, applications, etc., to achieve the effect of clear targets and strong specificity

Active Publication Date: 2018-12-14
HUNAN NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The present invention intends to provide a new protein with anti-breast cancer activity found in the egg grains of the spider spider. At present, there is no relevant report on the protein with anti-breast cancer activity in the egg grains of the spider spider

Method used

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  • Latrodectus tredecimguttatus egg granule protein with anti-breast cancer activity, and preparation method and application thereof
  • Latrodectus tredecimguttatus egg granule protein with anti-breast cancer activity, and preparation method and application thereof
  • Latrodectus tredecimguttatus egg granule protein with anti-breast cancer activity, and preparation method and application thereof

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Embodiment Construction

[0042] The invention will be further described below in conjunction with the embodiments and accompanying drawings, but they are not used to limit the scope of the invention.

[0043] Due to the limited source of E. coli eggs, the content of the anti-breast cancer active protein in eggs is not high, so it is difficult to directly extract and prepare samples from eggs. In the embodiment of the present invention, samples are prepared by heterologous expression of Escherichia coli .

[0044] 1. Cloning and heterologous expression of anti-breast cancer protein coding genes

[0045] (1) First, a series of open reading frames (ORFs) that might encode active proteins and polypeptides were obtained through the analysis of the transcriptome of E. According to the results of bioinformatics analysis, we selected one of the ORFs for gene cloning and heterologous expression, and the nucleotide sequence of the selected coding gene was as follows:

[0046]

[0047] The nucleotide sequence...

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Abstract

The invention provides a latrodectus tredecimguttatus egg granule protein with anti-breast cancer activity, and a preparation method and application thereof. The amino acid sequence of the protein isshown as SEQ ID NO:1, and the molecular weight of the protein is 10172.2 Da. The protein can be prepared by the steps of acquiring a coding gene from a latrodectus tredecimguttatus egg granule particle transcriptome and then carrying out heterologous expression by using escherichia coli. The protein can specifically act on MDA-MB-231 cells in a human breast cancer cell line, inhibit the proliferation and metastasis of the MDA-MB-231 cells, and induce apoptosis of the MDA-MB-231 cells, so that an effect of resisting breast cancer is achieved. The protein can be applied to preparation of drugs for inhibiting growth of the MDA-MB-231 cells, can be used as a precursor molecule for preparing drugs for treating breast cancer, and can be combined with other anti-tumor drugs for preparing drugs for treating breast cancer. The protein with anti-breast cancer activity can inhibit breast cancer cells from a plurality of aspects, thus not only can be used as a precursor molecule for the development of drugs for treating breast cancer, but also can exert an effect in related researches such as tumor generation and development mechanisms and the like, and has a valuable application prospect.

Description

technical field [0001] The present invention relates to a protein with anti-breast cancer activity and its preparation method and application, especially relates to a protein capable of inhibiting the proliferation and metastasis of human breast cancer cell line MDA-MB-231 and inducing its apoptosis The egg granule protein of the spotted spider and its preparation method and application. Background technique [0002] Female breast cancer is one of the most common malignant cancers in the world. The risk of breast cancer generally increases with age, which seriously threatens women's health and even life safety. The incidence of breast cancer is increasing, which is associated with increasing life expectancy, increasing urbanization and the widespread adoption of Western lifestyles. In China, the incidence of breast cancer is 11.61 / 100,000, which is relatively high. At the same time, due to the accurate screening, diagnosis, intervention and effective treatment of breast ca...

Claims

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Application Information

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IPC IPC(8): C07K14/435C12N15/12C12N15/70A61K38/17A61P35/00A61P15/14
CPCA61K38/00A61P15/14A61P35/00C07K14/43518C12N15/70
Inventor 王贤纯徐德宏
Owner HUNAN NORMAL UNIVERSITY
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