Enzyme-free glucose electrochemical sensing electrode

Abstract

The invention discloses an enzyme-free glucose electrochemical sensing electrode. The technical scheme of the invention uses foamed Ni as a template to mass-synthesize a three-dimensional graphene material 3D-G-Ni by a vapor deposition method (CVD). The enzyme-free glucose electrochemical sensing electrode with high sensitivity is established by directly using the 3D-G-Ni as an electrode materialand a sensing interface, which is used for the determination of glucose concentration in human serum. Through various investigations, the following results are obtained, in the 0.1M of a NaOH solution, the correlation data is determined by using an optimal potential of 0.50V, a linear range of glucose concentration measured by the 3D-G-Ni electrode is wide, which is in accordance with different linear equations at 10-1000 [mu]M, 1000-4000 [mu]M, and 4000-10000 [mu]M respectively, and the detection limit is 1 [mu]M. The glucose concentration measured by the 3D-G-Ni electrode serum is close to ahospital data, the change of the values is little, and the accuracy and precision are high. An interfering substance has less influence on the measurement results, and has better anti-interference. The same electrode can be used multiple times in one day, and the measurement data has good reappearance. The results of measuring the same sample with different electrodes are good, and the differencebetween the electrodes is small.

Description

technical field [0001] The invention belongs to the field of electrochemical sensors, and specifically refers to a high-sensitivity three-dimensional disposable enzyme-free glucose electrochemical sensing electrode. Background technique [0002] The monitoring of blood glucose concentration in diabetic patients is very important for the prevention and control of diabetes. Among all kinds of glucose monitoring methods, the electrochemical method has become the most popular in the development of portable glucose sensors due to its outstanding advantages of simple device, fast analysis speed, high sensitivity, good precision, easy operation, and especially the ease of miniaturization of instruments and devices. A popular research direction, and produced a series of commercial electrochemical blood glucose measuring instruments. [0003] The core technology of electrochemical glucose sensor lies in the preparation of highly sensitive sensing electrodes. The first glucose sensi...

AI Technical Summary

Problems solved by technology

The three generations of glucose enzyme sensing electrodes rely on the catalysis of GOX on the electrode surface when responding to the signal, but the activity of the enzyme is easily affected by temperature, humidity and pH; affect the accuracy of the measurement

[0006] Although new materials for glucose-free electrochemical sensing electrodes are constantly emerging, in the development of enzyme-free glucose electrochemical sensing electrodes, nanomaterials themselves are difficult to be used as electrode matrix materials, and nanomaterials must be used in various ways. Modified to the surface of commercial support electrodes such as glassy carbon electrodes, gold electrodes, and ITO, the preparation process of sensing electrodes is complicated, and nanomaterials are easy to agglomerate or fall off during the fixation process, resulting in large batch-to-batch differences in electrodes

After the supporting electrode is used, it must be re-polished, modified, etc., and the batch preparation and application of the electrode cannot be realized.

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