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A kind of extraction method of bradyrhizobium extract and its application on mango grafting

A technology of bradyrhizobium and extract, which is applied in the field of fruit tree planting, can solve the problems of low survival rate of grafting, unsatisfactory healing effect of grafting interface, and low utilization rate of bradyrhizobium research, so as to promote scab healing and healing The effect of promoting growth and development in a short period of time

Active Publication Date: 2022-01-28
云南元江大有为食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention overcomes the low utilization rate of bradyrhizobium in the prior art, the unsatisfactory effect of the existing plant healing agent on promoting the healing of the grafting interface, and the low survival rate of grafting, and provides a kind of bradyrhizobium extract. Extraction method and its application in mango grafting

Method used

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  • A kind of extraction method of bradyrhizobium extract and its application on mango grafting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for extracting Bradyrhizobium extract, comprising the steps of:

[0020] (1) Selection and cultivation of Bradyrhizobium: select Bradyrhizobium from the roots of peanuts, then dry and pulverize the peanut roots, use ethanol for rough extraction to obtain crude extracts, and then perform supercritical carbon dioxide extraction on the crude extracts, The crude extract is placed in a supercritical extraction ax for supercritical extraction, and an equal mass ratio of ethanol, ethyl acetate, and n-butanol mixture is used as an entrainer to extract for 20 minutes to obtain the Bradyrhizobium extract; the supercritical The carbon dioxide extraction temperature is 35°C, the extraction pressure is 35kpa, and the carbon dioxide flow rate is 25L / min. The selected bradyrhizobium was placed in the culture medium, cultured at 28° C. for 5 days to obtain a culture solution, and the culture solution was filtered and sterilized by gauze to obtain a bradyrhizobium fermentation ...

Embodiment 2

[0023] A method for extracting Bradyrhizobium extract, comprising the steps of:

[0024] (1) Selection and cultivation of Bradyrhizobium: select Bradyrhizobium from the roots of peanuts, then dry and pulverize the peanut roots, use ethanol for rough extraction to obtain crude extracts, and then perform supercritical carbon dioxide extraction on the crude extracts, The crude extract is placed in a supercritical extraction ax for supercritical extraction, and an equal mass ratio of ethanol, ethyl acetate, and n-butanol mixture is used as an entrainer to extract for 20 minutes to obtain the Bradyrhizobium extract; the supercritical The carbon dioxide extraction temperature is 35°C, the extraction pressure is 35kpa, and the carbon dioxide flow rate is 25L / min. The selected bradyrhizobium was placed in the culture medium, cultured at 25° C. for 6 days to obtain a culture solution, and the culture solution was filtered and sterilized by gauze to obtain a bradyrhizobium fermentation ...

Embodiment 3

[0027] A method for extracting Bradyrhizobium extract, comprising the steps of:

[0028] (1) Selection and cultivation of Bradyrhizobia: select Bradyrhizobium from the roots of peanuts, then dry and pulverize the peanut roots, use ethanol for rough extraction to obtain crude extracts, and then carry out supercritical carbon dioxide extraction of the crude extracts, The crude extract is placed in a supercritical extraction ax for supercritical extraction, and an equal mass ratio of ethanol, ethyl acetate, and n-butanol mixture is used as an entrainer to extract the bradyrhizobium extract for 20 minutes; the supercritical The carbon dioxide extraction temperature is 35°C, the extraction pressure is 35kpa, and the carbon dioxide flow rate is 25L / min. The selected bradyrhizobium was placed in the culture medium, cultured at 26° C. for 5 days to obtain a culture solution, and the culture solution was filtered and sterilized by gauze to obtain a bradyrhizobium fermentation broth; th...

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Abstract

The invention discloses an extraction method of a bradyrhizobium extract and its application in mango grafting. The extraction method is as follows: (1) Selection and cultivation of Bradyrhizobium: select Bradyrhizobium from the roots of peanuts, then dry and crush the peanut roots, use ethanol for rough extraction to obtain crude extracts, and then carry out ultra- Critical carbon dioxide extraction, the crude extract is placed in a supercritical extraction ax for supercritical extraction to obtain the bradyrhizobium extract; the selected bradyrhizobium is cultured in a medium to obtain a culture solution, and the culture medium is cultured with gauze The bradyrhizobium fermentation liquid is obtained after the liquid is filtered and sterilized; the bradyrhizobium extract is mixed with the bradyrhizobium fermentation liquid to obtain the bradyrhizobium extract. The invention adopts the bradyrhizobium extract and the bradyrhizobium fermented liquid to mix to prepare the agent for promoting grafting wound healing, which can promote the healing of mango after grafting, has short healing time of grafting interface and high grafting survival rate.

Description

technical field [0001] The invention relates to the technical field of fruit tree planting, in particular to a method for extracting a bradyrhizobium extract and its application in mango grafting. Background technique [0002] At present, grafting technology is widely used in horticultural crops. In production practice, the factors affecting the survival of grafted seedlings are not only the affinity of rootstock and ear, but also the nutritional status of rootstock and ear, moisture, sterilization and other factors, which affect the healing speed of grafted joints. In order to ensure the survival rate of grafting, people apply various chemical substances to the grafting to promote the healing of the grafting wound as soon as possible, and the effect is more obvious. In the existing research, the components that have been grafted into the interface healing agent mainly contain plant growth regulators such as naphthalene acetic acid, indole acetic acid and gibberellin, bacte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G2/30A01G2/38A01G7/06C12N1/20C12R1/41
CPCA01G2/30A01G2/38A01G7/06C12N1/20
Inventor 韦绍仙
Owner 云南元江大有为食品有限公司
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