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Auxiliary premature birth diagnosis marker based on mixed exposure detection of chromium and arsenic and application thereof

A technology for auxiliary diagnosis and biomarkers, applied in the fields of analytical chemistry and clinical medicine, can solve problems such as lack of corresponding attention, and achieve the effects of delaying and preventing disease progression, simple sample processing, and rapid and accurate instrument analysis.

Inactive Publication Date: 2019-01-08
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of ICP-MS to analyze the mixed exposure level of arsenic and chromium in whole blood during pregnancy has not received corresponding attention in the auxiliary diagnosis of preterm birth.

Method used

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  • Auxiliary premature birth diagnosis marker based on mixed exposure detection of chromium and arsenic and application thereof
  • Auxiliary premature birth diagnosis marker based on mixed exposure detection of chromium and arsenic and application thereof
  • Auxiliary premature birth diagnosis marker based on mixed exposure detection of chromium and arsenic and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Embodiment 1 Research Object Selection and Grouping Basis

[0081] The inventor collected whole blood samples from pregnant women who met the requirements from Nanjing Maternal and Child Health Hospital Affiliated to Nanjing Medical University. After the first stage of follow-up, according to the birth outcome, pregnant women with premature infants were included in the case group (100 people), and 100 people were matched by age and BMI as controls. A total of 200 people were used as experimental subjects for the assessment of mixed exposure of premature babies to arsenic and chromium. The specific sample classification criteria are as follows:

[0082] The first stage of association exploration

[0083] 100 cases were included, pregnant mothers of premature infants; 100 pregnant mothers of full-term infants were matched according to age and BMI.

[0084] Group A: healthy control group (100 people, with premature babies):

[0085] 1. Age between 20-35 years old

[00...

Embodiment 2

[0094] Example 2 ICP-MS detection of mixed exposure of arsenic and chromium and analysis of preterm birth

[0095] 1. Sample pretreatment

[0096] Take 100 μl of whole blood, add 4.9ml of reagent A (that is, dilute 50 times), and mix well.

[0097] 2. Instrument testing

[0098] Analytical instrument: ICAP RQ ICP-MS (Thermo)

[0099] a. After ICP-MS is ignited and started, it is stable for about half an hour; pump 1 ppb tuning solution (provided by the instrument manufacturer, Ba, Bi, Ce, Co, Ln, Li, U, each concentration is 1 μg / L, and the matrix is ​​2.5 %HNO 3 , 0.5% HCl), according to the sensitivity of Li, In and U, the oxide (CeO) and the double charge (Ba 2+ ) level, tune the entire mass range (4 ~ 290amu) and achieve the best state; tuning requirements meet the following standards: standard mode sensitivity 7Li≥4000cps / ppb, 115In≥15000cps / ppb; 238U≥20000cps / ppb; standard mode Double charged Ba 2+ / Ba<3.0%; standard mode oxide 156CeO / 140Ce<2.0%; optimized operatin...

Embodiment 3

[0113] Example 3 Production of a kit for detecting the mixed exposure of arsenic and chromium in the whole blood of pregnant women to assist in the diagnosis of premature birth

[0114]Firstly, the ICP-MS method was used to determine the high levels of arsenic and chromium concentrations in the whole blood of the case group, and then the Chi-square test was used to find that there were significant differences in the composition ratios of individuals with different exposure levels between the healthy control group and the case group. The number of people with high exposure was more than that of the control group, and high exposure to arsenic and chromium significantly increased the incidence of preterm birth. Detection of mixed exposure to arsenic and chromium can be used for auxiliary diagnosis of preterm birth. This kit includes a batch of reagents and consumables for the detection of arsenic and chromium in whole blood. The diluent is used for sample dilution, which contains...

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Abstract

The invention belongs to the field of analytical chemistry and clinical medicine, and discloses an auxiliary premature birth diagnosis marker based on mixed exposure detection of chromium and arsenicand application thereof. An exposure biomarker related to auxiliary premature birth diagnosis is based on mixed exposure of the chromium and the arsenic. The auxiliary premature birth diagnosis markerdetects the concentrations of the arsenic and the chromium in whole blood by ICP-MS, can be applied to auxiliary diagnosis of premature birth, is low in blood consumption and simple, fast and accurate in operation, and has a relatively good clinical popularization value.

Description

field of invention [0001] The invention belongs to the field of analytical chemistry and clinical medicine, and relates to an exposure biomarker related to auxiliary diagnosis of premature birth and its detection method and application. The exposure biomarker is mixed exposure of arsenic and chromium, and the detection method is based on ICP-MS Detection method. Background technique [0002] Preterm birth (PTD) is the leading cause of neonatal and child mortality worldwide, the second leading cause of death in children under 5 years of age, and the most important direct cause of neonatal death in the first month, and has become a major contributor to the global burden of disease one of the risk factors. Preterm babies refer to newborns born between 28 weeks and less than 37 weeks of gestation. The incidence of premature babies in the world is 9.1-13.4%, and the incidence of premature babies in low-income countries is higher (11.8%). For example, the premature birth rate in ...

Claims

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Application Information

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IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 陈瑛杨旭杨蕊匡华龙陈爱玲夏彦恺陈敏健王心如
Owner NANJING MEDICAL UNIV
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