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ICP-MS-based (inductively coupled plasma-mass spectrometry-based) mercury and arsenic mixed exposure test low-birth-weight auxiliary diagnosis marker and application thereof

An ICP-MS, low birth weight technology, applied in the fields of analytical chemistry and clinical medicine, can solve the problems of undeveloped fetal growth and development, adverse effects on fetal development, unfavorable fetuses, etc., to delay and prevent disease progression, rapid and accurate instrument analysis , The effect of preventing sample contamination

Inactive Publication Date: 2019-03-12
ZHEJIANG CELLPRO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Existing studies have shown that individual exposure to mercury and arsenic will have varying degrees of adverse effects on fetal development, but studies on fetal growth and development with mixed exposure to mercury and arsenic have hardly been carried out.
It is still unknown whether mixed exposure to mercury and arsenic will have a synergistic or additive effect, making mixed exposure more toxic than single exposure, and having more adverse effects on the fetus

Method used

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  • ICP-MS-based (inductively coupled plasma-mass spectrometry-based) mercury and arsenic mixed exposure test low-birth-weight auxiliary diagnosis marker and application thereof
  • ICP-MS-based (inductively coupled plasma-mass spectrometry-based) mercury and arsenic mixed exposure test low-birth-weight auxiliary diagnosis marker and application thereof
  • ICP-MS-based (inductively coupled plasma-mass spectrometry-based) mercury and arsenic mixed exposure test low-birth-weight auxiliary diagnosis marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Embodiment 1 Research Object Selection and Grouping Basis

[0069] The inventors collected whole blood samples from low birth weight cases and normal control groups that met the requirements from Nanjing Maternal and Child Health Hospital Affiliated to Nanjing Medical University. Those with normal birth weight were regarded as controls, and those with low birth weight were regarded as cases. A total of 111 cases were randomly included, and 111 healthy controls were matched according to maternal age and BMI. A total of 222 people were used as experimental subjects for the mixed exposure assessment of mercury and arsenic with low birth weight. The specific sample classification criteria are as follows:

[0070] The first stage of association exploration

[0071] A total of 111 case infants were included, and 111 matched controls were matched according to mother's age and BMI, totaling 222.

[0072] Group A: healthy control group (111 people, normal birth weight):

[0...

Embodiment 2

[0082] Example 2 Analysis of ICP-MS detection of mixed exposure to mercury and arsenic and risk of low birth weight

[0083] 1. Sample pretreatment

[0084] Take 100 μl of whole blood, add 4.9ml of reagent A (that is, dilute 50 times), and mix thoroughly.

[0085] 2. Instrument testing

[0086] 1) Analytical instrument: ICAP RQ ICP-MS (Thermo)

[0087] 2) Instrument conditions: After the ICP-MS is ignited and started, it is stable for about half an hour. Pump 1ppb tuning solution, according to the sensitivity of Li, In and U, oxide (CeO) and double charge (Ba 2+ ) level, tune the entire mass range (4-290 amu) and reach the best state. Tuning requirements meet the following standards: standard mode sensitivity 7Li≥4000 cps / ppb, 115In≥15000cps / ppb; 238U≥20000cps / ppb; standard mode double charge Ba 2+ / Ba<3.0%; standard mode oxide 156CeO / 140Ce<2.0%.

[0088] 3) Recommended parameters for ICP-MS

[0089]

[0090]

[0091] 4) Sampling method: automatic sampling, sampli...

Embodiment 3

[0098] Example 3 Production of a kit for detecting mixed exposure to mercury and arsenic in male whole blood to aid in the diagnosis of low birth weight

[0099] Firstly, the ICP-MS method was used to determine the high levels of mercury and arsenic concentrations in the whole blood of the case group (the exposure level was above the 75% quantile of the total sample), and then it was found that the healthy control group was different from the case group through the Chi-square test. There were significant differences in the composition ratio of individuals with exposure levels. The number of people with high exposure in the case group was more than that in the control group. High exposure to mercury and arsenic significantly increased the risk of low birth weight. Detection of mixed exposure to mercury and arsenic can be used for auxiliary diagnosis of low birth weight. This kit includes a batch of reagents and consumables for the detection of mercury and arsenic in whole blood...

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Abstract

The invention discloses an ICP-MS-based mercury and arsenic mixed exposure test low-birth-weight auxiliary diagnosis marker and application thereof and belongs to the fields of analytic chemistry andclinical medicine. The ICP-MS-based mercury and arsenic mixed exposure test low-birth-weight auxiliary diagnosis marker, namely, a low-birth-weight related exposure biomarker, is a mixture of mercuryand arsenic and applies an ICP-MS-based detecting method. Comparison of the mercury and arsenic mixed exposure levels in normal contrast whole blood and the whole blood of a low-birth-weight case through ICP-MS helps discover that man's whole blood contains a marker combination applicable to assessing risk low-birth-weight and achieving auxiliary diagnosis values. The invention also relates to application of ICP-MS of detection of the ICP-MS-based mercury and arsenic mixed exposure test low-birth-weight auxiliary diagnosis marker to help develop a low-birth-weight auxiliary diagnosis kit applicable to clinical application.

Description

field of invention [0001] The invention belongs to the field of analytical chemistry and clinical medicine, and relates to an exposure biomarker related to low birth weight and its detection method and application. The exposure biomarker is mercury and arsenic mixed exposure, and the detection method is based on ICP-MS detection method. Background technique [0002] Low birth weight is an important public health problem facing the world today. According to the statistics of WHO in 2010, the proportion of low birth weight newborns in the world is 15% on average, and the highest areas are Pakistan and Yemen, as high as 32%. According to the survey, the average incidence of low birth weight in newborns in my country is about 4.6%, and the highest reaches 6.3%. Although the proportion is relatively low, my country has a large population base, and the number of low birthweight infants cannot be underestimated. Low birthweight is still a prominent problem restricting the health ...

Claims

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Application Information

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IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 薛志刚胡西陵
Owner ZHEJIANG CELLPRO BIOTECH
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