Application of a marker for auxiliary diagnosis of macrosomia

A marker and a huge technology, applied in the fields of analytical chemistry and clinical medicine, can solve the problems of complex experimental steps and low sensitivity, and achieve the effects of simple sample processing, high sensitivity and specificity, and rapid and accurate instrument analysis

Active Publication Date: 2016-05-18
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

NMR technology is characterized by no damage to the components to be measured, simple sample pretreatment, but low sensitivity; gas chromatography-mass spectrometry has good sensitivity and reproducibility, but derivatization methods are generally used for sample pretreatment , making the experimental steps more complicated

Method used

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  • Application of a marker for auxiliary diagnosis of macrosomia
  • Application of a marker for auxiliary diagnosis of macrosomia
  • Application of a marker for auxiliary diagnosis of macrosomia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Embodiment 1: Research object selection and group basis

[0084] The present inventor collected blood samples from mothers of macrosomia patients and normal children who met the requirements from the Nanjing Maternal and Child Health Hospital Affiliated to Nanjing Medical University (see figure 1 , figure 2 ). Weigh the newborn after birth, and if the weight is equal to or greater than 4kg within 1 hour after birth, it is diagnosed as "macrosomia". In the first stage, 100 samples of pregnant women who met the requirements were randomly included, 15 of which were macrosomia; in the second stage, 30 samples of pregnant women were included, of which 15 were compared with 15 cases of macrosomia. Screening subjects for markers. Specific sample classification criteria are as follows:

[0085] first stage screening

[0086] A total of 100 pregnant women were randomly included.

[0087] 1. Between 23 and 36 years old;

[0088] 2. No gestational hypertension, gestational...

Embodiment 2

[0110] Example 2: UPLC-MS Metabolomics Biomarker Screening for Macrosomia

[0111] 1. Sample pretreatment

[0112] 1.1. Fresh pregnant blood (100 samples in the screening stage) was centrifuged in a centrifuge at 3000 rpm for 5 minutes, and 100 μl of the supernatant was taken and dispensed into clean 1.5ml EP tubes.

[0113] 1.2.50 μl of plasma was precipitated with 150 μl of methanol (reagent C).

[0114] 1.3. Aspirate the supernatant, blow dry with nitrogen and then vacuum dry.

[0115] 1.4. Dissolve the dry matter with 100 μL of water (reagent D).

[0116] 2. Instrument testing

[0117] 2.1. Analytical instruments: UPLCUltimate3000system (Dionex) high-performance liquid chromatography; Q-Exactive high-resolution mass spectrometer.

[0118] 2.2. Liquid phase conditions:

[0119] 2.2.1 The liquid chromatographic column is a C18 chromatographic column (100mm×2.1mm, particle size 1.9μm), and the column temperature is 40°C. The preferred liquid chromatography column is a Hyp...

Embodiment 3

[0132] Example 3: Stability Analysis of Plasma Metabolic Small Molecules

[0133] The same collection method as in Example 1 was used to collect plasma from nine mothers during pregnancy, and the method in Example 2 was used to evaluate the stability of plasma levels of nicotinamide and citric acid (the interval was 2 weeks). The results showed that the measured levels of nicotinamide and citrate in plasma were stable ( Figure 4 ), with properties as diagnostic / monitoring markers.

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Abstract

The invention belongs to the field of analytic chemistry and clinical medicine and discloses a fetal macrosomia auxiliary diagnostic marker and application thereof. The marker is the combination of nicotinamide and citric acid and can be used for manufacturing a fetal macrosomia diagnosing or monitoring kit. The marker has the better auxiliary diagnostic effect on fetal macrosomia, can lower the detection cost and reduce economical burdens of a patient and is beneficial to clinical usage and popularization.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and clinical medicine, and relates to an auxiliary diagnostic marker for macrosomia and its application. Background technique [0002] Macrosomia refers to newborns whose weight is greater than or equal to 4kg within 1 hour after birth. The occurrence of macrosomia is harmful to both mother and baby. Among women in my country, the dystocia mortality rate caused by macrosomia is higher than the mortality rate of normal birth; wound infection, abdominal adhesions, endometriosis and other diseases caused by macrosomia after cesarean section may directly or indirectly lead to maternal death . Macrosomia is also harmful to the health of newborns. Macrosomia is prone to bone damage through forced traction during delivery, and suffocation and even death may occur due to prolonged delivery time; macrosomia after cesarean section is prone to hypoglycemia, polycythemia, and high biliary redness Hyperg...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 陈敏健董天宇吴克勤何晓伟夏彦恺江华胡艳辉吴炜陆春城王心如
Owner NANJING MEDICAL UNIV
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