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Marker and kit for diagnosing early esophageal cancer

A marker and kit technology, which is applied in the field of markers and kits for diagnosing early esophageal cancer, can solve the problems of limiting the promotion of endoscopic screening, trauma and high cost of endoscopic screening, and achieves good diagnostic effect and differentiation. The effect is good and the detection rate is improved

Pending Publication Date: 2021-12-10
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, endoscopic screening is invasive, costly, and inefficient, which limits the promotion of endoscopic screening in the early detection of esophageal cancer in asymptomatic populations.

Method used

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  • Marker and kit for diagnosing early esophageal cancer
  • Marker and kit for diagnosing early esophageal cancer
  • Marker and kit for diagnosing early esophageal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Screening of serum differential metabolic markers for esophageal cancer

[0036] 1. Experimental samples

[0037] According to strict screening and exclusion criteria, 100 age- and sex-matched healthy subjects (normal control group) and 100 patients with esophageal cancer (esophageal cancer group) were collected from the First Affiliated Hospital of Zhengzhou University.

[0038] The inclusion criteria for healthy subjects were: (1) normal persons between the ages of 40 and 79 who underwent physical examination in the hospital or family members accompanying the patients; (2) no abnormal hyperplasia of the esophageal epithelium.

[0039] Exclusion criteria for healthy subjects: (1) have taken penicillin, tetracycline, sulfonamides, quinolones and other antibiotics in the past year; (2) have other tumors, cardiovascular and cerebrovascular diseases, schizophrenia, senile dementia, digestive Ulcers, gastrointestinal polyps and other important organ dysfunction;...

Embodiment 2

[0072] Example 2: Evaluation of the ability of differential metabolite diagnostics to differentiate esophageal cancer patients from healthy individuals

[0073] 1. The ability of single differential metabolite diagnosis to distinguish esophageal cancer patients from normal people:

[0074] Hexyl alcohol, malonic acid, sebacic acid in the esophageal cancer group (100 routine esophageal cancer patients) and the normal control group (100 routine healthy subjects) serum samples detected based on UPLC-Q / TOF-MS in embodiment 1 The content data were analyzed, and the receiver operating curve (ROC curve) was used to evaluate the ability of each differential metabolite to diagnose and distinguish esophageal cancer patients from normal people. The ROC curves of esophageal cancer patients and normal people in the diagnosis of hexanehexanol, malonic acid and sebacic acid alone are as follows: figure 2 , image 3 , Figure 4 shown. According to the ROC curve, the area under the curve...

Embodiment 3

[0094] Example 3: Application of three differential metabolites in the screening of esophageal cancer

[0095] 1. Collection of serum samples

[0096] According to strict screening and exclusion criteria, 200 healthy subjects (normal control group) and 200 patients with esophageal cancer (esophageal cancer group) were collected from the First Affiliated Hospital of Zhengzhou University after age and sex matching.

[0097] The inclusion criteria for healthy subjects were: (1) normal persons between the ages of 40 and 79 who underwent physical examination in the hospital or family members accompanying the patients; (2) no abnormal hyperplasia of the esophageal epithelium.

[0098] Exclusion criteria for healthy subjects: (1) have taken penicillin, tetracycline, sulfonamides, quinolones and other antibiotics in the past year; (2) have other tumors, cardiovascular and cerebrovascular diseases, schizophrenia, senile dementia, digestive Ulcers, gastrointestinal polyps and other imp...

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Abstract

The invention belongs to the field of disease detection and diagnosis, and particularly discloses a marker and a kit for diagnosing early esophageal cancer. The marker for diagnosing the early esophageal cancer is at least one of hexanehexol, malonic acid and sebacic acid, and a detection reagent of the marker can be used for preparing an early esophageal cancer diagnosis product. The invention further provides the kit for diagnosing the early esophageal cancer, the kit contains the detection reagent for detecting the mentioned marker, and the detection reagent is a reagent for detecting the marker in a sample through chromatography, mass spectrometry or chromatography-mass spectrometry. The esophageal cancer can be effectively detected by detecting the expression levels of hexanehexol, malonic acid and sebacic acid in human serum, the detection sensitivity is as high as 84%, the specificity reaches 76%, and the kit and the marker can be used for large-scale screening of asymptomatic crowds in esophageal cancer high-incidence areas and are beneficial to screening and early discovery of asymptomatic high-risk crowds.

Description

technical field [0001] The invention relates to the field of disease detection and diagnosis, in particular to a marker and a kit for diagnosing early esophageal cancer. Background technique [0002] Esophageal cancer is one of the most common malignant tumors of the digestive system worldwide, and its death rate ranks sixth. In my country, the incidence of esophageal cancer is not only high, but also has significant regional differences. The 5-year survival rate of patients with advanced esophageal cancer is only about 10%, while that of early-stage esophageal cancer can reach more than 90%. Due to the lack of obvious specific symptoms in the early stage of esophageal cancer patients, and the lack of economical, efficient and sensitive biomarkers suitable for the screening of large-scale high-risk groups, most of the esophageal cancer patients who are currently clinically diagnosed are in the middle and late stages stage. [0003] Since the onset of esophageal cancer is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N27/62
CPCG01N30/02G01N27/62
Inventor 宋昕王立东唐来仪魏梦霞韩文莉雷玲玲赵学科王盼盼杨苗苗胡景峰钟侃徐瑞华李贝
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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