Combination of capric acid and prostaglandin e2 as auxiliary diagnostic markers for macrosomia and its application

An auxiliary diagnosis and prostaglandin technology, which is applied in the field of analytical chemistry and clinical medicine, can solve the problems of complex experimental steps and low sensitivity, and achieve the effects of improving sensitivity and specificity, easy detection, delaying and preventing disease progression

Active Publication Date: 2020-08-21
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

NMR technology is characterized by no damage to the components to be measured, simple sample pretreatment, but low sensitivity; gas chromatography-mass spectrometry has good sensitivity and reproducibility, but derivatization methods are generally used for sample pretreatment , making the experimental steps more complicated

Method used

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  • Combination of capric acid and prostaglandin e2 as auxiliary diagnostic markers for macrosomia and its application
  • Combination of capric acid and prostaglandin e2 as auxiliary diagnostic markers for macrosomia and its application
  • Combination of capric acid and prostaglandin e2 as auxiliary diagnostic markers for macrosomia and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Embodiment 1 Research Object Selection and Grouping Basis

[0094] The present inventor collected blood samples from mothers of macrosomia patients and normal children who met the requirements from the Nanjing Maternal and Child Health Hospital Affiliated to Nanjing Medical University (see figure 1 , figure 2 ). Weigh the newborn after birth, and if the weight is equal to or greater than 4kg within 1 hour after birth, it is diagnosed as "macrosomia". In the first stage, 97 samples of pregnant women who met the requirements were randomly included, 15 of which were macrosomia; in the second stage, 30 samples of pregnant women were included, 15 of which were compared with 15 cases of macrosomia, as the blood metabolism of small molecular organisms during pregnancy of macrosomia. Screening subjects for markers. Specific sample classification criteria are as follows:

[0095] first stage screening

[0096] A total of 97 pregnant women were randomly included.

[0097] ...

Embodiment 2

[0110] Example 2: UPLC-MS Metabolomics Biomarker Screening for Macrosomia

[0111] 1. Sample pretreatment

[0112] 1.1. Centrifuge fresh pregnant blood at 3000rpm for 5min, take 100μL supernatant and put it into a clean 1.5ml EP tube.

[0113] 1.2. Add 50 μL of water to 50 μL of supernatant and add 300 μL of methanol (reagent A) to precipitate protein.

[0114] 1.3. Aspirate the supernatant, blow dry with nitrogen and then vacuum dry.

[0115] 1.4. Reconstitute with 20 μL of the dried product (reagent D).

[0116] 2. Instrument testing

[0117] 2.1. Analytical instruments: UPLC Ultimate 3000 system (Dionex) high-performance liquid chromatography; Q-Exactive high-resolution mass spectrometer.

[0118] 2.2. Liquid phase conditions:

[0119] 2.2.1. The liquid chromatographic column is a Hypersil GOLD C18 chromatographic column (100mm×2.1mm, particle size 1.9μm, Thermo Scientific, Germany), and the column temperature is 40°C.

[0120] 2.2.2 Mobile phase A is water containing...

Embodiment 3

[0132] Example 3 Stability Analysis of Blood Metabolism Small Molecules

[0133] The method of Example 2 was used to evaluate the stability of the combination level of capric acid and prostaglandin E2 in the mother's blood during pregnancy (the interval was 2 weeks). The results showed that combined capric acid and prostaglandin E2 levels in blood were stable ( Figure 4 ), with properties as diagnostic / monitoring markers.

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Abstract

The invention belongs to the fields of analytical chemistry and clinical medicine, and discloses a capric acid and prostaglandin E2 combination as a giant baby auxiliary diagnosis marker and the application thereof. The auxiliary diagnosis marker is a combination of micromolecular capric acid and prostaglandin E2, can be used for preparing a giant baby auxiliary diagnosis or monitoring kit, is relatively high in sensitivity and specificity and rapid in detection, and has a relatively high clinical application value.

Description

[0001] field of invention [0002] The invention belongs to the fields of analytical chemistry and clinical medicine, and relates to a combination of capric acid and prostaglandin E2 as an auxiliary diagnostic marker for macrosomia and its application. Background technique [0003] Macrosomia refers to newborns whose weight is greater than or equal to 4kg within 1 hour after birth. The occurrence of macrosomia is harmful to both mother and baby. Macrosomia is one of the important factors of dystocia, and it also increases the risk of heart abnormalities. Studies have now found that macrosomia children have a higher chance of suffering from obesity when they grow up, and will become susceptible to various diseases such as diabetes and high blood pressure. [0004] At present, the early diagnosis of macrosomia is generally based on some predisposing medical characteristics of the mother, such as diabetes, obesity and post-term pregnancy. However, for those who do not have the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/88
Inventor 陈敏健王圆媛张婷杨旭夏彦恺胡艳辉陈道帧周作民陆春城吴炜王心如
Owner NANJING MEDICAL UNIV
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