Method for measuring 25-hydroxyvitamin D
A hydroxyvitamin and biotinylation technology, applied in the field of detection of 25-hydroxyvitamin D in blood samples, can solve problems such as prolonging detection time, linear influence, and increasing detection steps.
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Embodiment 1
[0052] Embodiment 1 measures the comparison of the results of the same source serum and plasma samples
[0053] Materials and Reagents
[0054] Take serum / plasma (EDTA-K2) pairs from 20 subjects,
[0055] Mike Biological Co., Ltd. 25-hydroxyvitamin D master calibration standard 1 set,
[0056] Mike Biological Co., Ltd. 25-hydroxyvitamin D assay kit (chemiluminescence method) 2 boxes (batch number: 0417011, specification: 100 tests / box),
[0057] Mike Biological Co., Ltd. Washing Buffer (Product No.: IM4202458, Lot No.: 0417141)
[0058] Substrate Solution for Fully Automatic Immunoassay System (Enzymatic Chemiluminescent Substrate Solution (HRP)) of Mike Biological Co., Ltd. (Product No.: IM4202459, Batch No.: 0417031)
[0059] Mike Biological Co., Ltd. IS1200 automatic chemiluminescence immunoassay analyzer (equipment number: 1015-01024).
[0060] 25-Hydroxyvitamin D Assay Kit contains the following reagents:
[0061] The first reagent (R1): 0.2 mol / L acetic acid-sodium ...
Embodiment 2
[0073] The different addition amounts of the second magnetic beads of embodiment 2;
[0074] Materials and Reagents
[0075] The same serum / plasma (EDTA-K2) pair from 20 subjects as in Example 1,
[0076] Mike Biological Co., Ltd. 25-hydroxyvitamin D master calibration standard 1 set,
[0077] Mike Biological Co., Ltd. 25-Hydroxyvitamin D Assay Kit (chemiluminescence method) 3 boxes (batch number: 0417011, specification: 100 tests / box),
[0078] Mike Biological Co., Ltd. Washing Buffer (Product No.: IM4202458, Lot No.: 0417141)
[0079] Substrate Solution for Fully Automatic Immunoassay System (Enzymatic Chemiluminescent Substrate Solution (HRP)) of Mike Biological Co., Ltd. (Product No.: IM4202459, Batch No.: 0417031)
[0080] Mike Biological Co., Ltd. IS1200 automatic chemiluminescence immunoassay analyzer (equipment number: 1015-01024)
[0081] 25-Hydroxyvitamin D Assay Kit contains the following reagents:
[0082] The first reagent (R1): 0.2 mol / L acetic acid-sodium a...
Embodiment 3
[0093] Materials and Reagents
[0094] The same serum / plasma (EDTA-K2) pair from 20 subjects as in Example 1,
[0095] Mike Biological Co., Ltd. 25-hydroxyvitamin D master calibration standard 1 set,
[0096]Mike Biological Co., Ltd. 25-Hydroxyvitamin D Assay Kit (chemiluminescence method) 3 boxes (batch number: 0417011, specification: 100 tests / box),
[0097] Mike Biological Co., Ltd. Washing Buffer (Product No.: IM4202458, Lot No.: 0417141)
[0098] Substrate Solution for Fully Automatic Immunoassay System (Enzymatic Chemiluminescent Substrate Solution (HRP)) of Mike Biological Co., Ltd. (Product No.: IM4202459, Batch No.: 0417031)
[0099] Mike Biological Co., Ltd. IS1200 automatic chemiluminescence immunoassay analyzer (equipment number: 1015-01024)
[0100] 25-Hydroxyvitamin D Assay Kit contains the following reagents:
[0101] The first reagent (R1): 0.2mol / L acetic acid-sodium acetate buffer solution (PH 4.5), 0.2 mg / mL of streptavidin magnetic particles as the firs...
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