Breeding method by utilizing distant hybridization and method for constructing gene library

Abstract

The invention discloses a breeding method by utilizing distant hybridization and a method for constructing a gene library. The breeding method includes: 1) distant hybridization: performing interspecific distant hybridization on upland cotton, sea island cotton, wild cotton and Gossypium arboretum to obtain distant hybridization generations; 2) quick purification of the distant hybridization generations: with a hemigamy material being a female parent and the distant hybridization generations as a male parent, performing chromosome doubling to a haplobiont in the F1 generation for homozygosis;3) determining a core parent; 4) introduction, expression and screening of insecticidal gene: hybridizing the core parent and an insecticidal species to obtain F2 and BC1 generations, planting the generations, and performing screening by combination of field insect survey and kanamycin detection; 5) selective breeding of cottons in different species: according to the insecticidal character, disease resistant character, yielding ability, prematurity and fiber quality, comprehensively judging the species and classifying the species. The method can accelerate stabilization of the target characters and acquire the species with the target characters, and can be used for constructing the breeding gene library without different target characters and creating conditions for the selective breeding.

Description

technical field [0001] The invention relates to a breeding method, in particular to a breeding method utilizing distant hybridization and a gene bank construction method. Background technique [0002] Cotton in the cotton area of ​​the Yellow River Basin generally only has a growth period of 180 days, which is suitable for planting upland cotton varieties that mature earlier. It is the largest cotton production area in China and an important commercial cotton base. The region has sufficient heat, a suitable frost-free period, and better sunshine than the cotton area in the Yangtze River Basin. However, the early summer is more dry, the latent rain is more concentrated, and the precipitation is variable, which can easily lead to the fall of flower bolls. This problem is not easy to solve in production. In addition, the temporal and spatial distribution of meteorological elements in the cotton area of ​​the Yellow River Basin is uneven, the stability of precipitation is poor, ...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a breeding method using distant hybridization and a method for constructing a gene bank. This method solves the problem of poor quality of existing germplasm, can accelerate the stabilization of target traits, and obtain disease resistance, high quality, and early maturation. , high-yielding, and widely suitable varieties, and built a breeding gene bank with different main target traits such as disease resistance, high quality, early maturity, high yield, and widely suitable, to create conditions for the breeding of multiple types of varieties