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Vascular endothelial cell PINK1 acetylation inhibitor and application thereof

A vascular endothelium and acetylation technology, applied in the field of pharmaceutical compositions containing astaxanthin, can solve the problems of easily disturbed stability, vascular endothelial dysfunction and the like

Inactive Publication Date: 2019-01-15
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, under pathological conditions, the stability of the internal environment of vascular endothelial cells is easily disturbed, which eventually leads to vascular endothelial dysfunction.

Method used

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  • Vascular endothelial cell PINK1 acetylation inhibitor and application thereof
  • Vascular endothelial cell PINK1 acetylation inhibitor and application thereof
  • Vascular endothelial cell PINK1 acetylation inhibitor and application thereof

Examples

Experimental program
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Embodiment Construction

[0011] Separation of tissue mitochondrial protein and cytoplasmic protein, using tissue mitochondrial separation kit (Beyond, China) to separate mitochondrial protein and cytoplasmic protein in myocardial tissue, the specific steps are as follows:

[0012] 1. Treat myocardial tissue with mitochondrial isolation reagent A.

[0013] 2. The mice were anesthetized with pentobarbital, the chest cavity was opened to cut the left ventricular myocardium, the cut tissue was weighed in a 1.5ml centrifuge tube, and the weight was recorded. Myocardial tissue was then washed once with pre-cooled PBS.

[0014] 3. Put the myocardial tissue in the EP tube, put the EP tube on ice, and then cut the myocardial tissue with scissors.

[0015] 4. Then add 10 volumes of pre-cooled PBS, and ice-bath for 3 minutes.

[0016] 5. Put it in a centrifuge, centrifuge at 600rpm for 10-20s, discard the supernatant.

[0017] 6. Add 8 volumes of pre-cooled trypsin digestion solution, ice bath for 20 minutes,...

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PUM

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Abstract

The invention discloses a pharmaceutical composition capable of inhibiting acetylation of PINK1 of vascular endothelial cells, which is characterized in that astaxanthin is contained and also discloses the use of the pharmaceutical composition in the preparation of medicaments for preventing and / or treating cardiovascular diseases including, but not limited to, atherosclerosis, coronary heart disease, angina pectoris, cerebral thrombosis, hypertension, hyperglycemia, hyperlipidemia, myocardial infarction, ischemic cor pulmonale and the like.

Description

technical field [0001] The invention relates to a pharmaceutical composition capable of inhibiting the acetylation of PINK1 in vascular endothelial cells, in particular to a pharmaceutical composition containing astaxanthin, which can inhibit the acetylation of PINK1 in vascular endothelial cells. Background technique [0002] With the deepening of research in recent years, vascular endothelial cells are no longer considered a simple barrier, but a complex organ. These cells play an extremely important role in maintaining blood vessel stability, and various regulators secreted by endothelial cells are necessary factors for regulating vascular tone, blood viscosity, immune response and cell growth. Nitric oxide synthase (eNOS) contained in endothelial cells catalyzes the production of nitric oxide (NO). Once NO is released into the blood, it acts on adjacent smooth muscle cells and activates guanylate cyclase to convert GTP into cGMP. cGMP-dependent Further activation of pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/122A61P9/10A61P9/12A61P3/10A61P3/06
CPCA61K31/122A61P3/06A61P3/10A61P9/10A61P9/12
Inventor 步世忠陈晓敏方亮
Owner NINGBO UNIV
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