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Discrete type biological sensing detection method for heavy metals in industrial products

A detection method and biosensing technology, applied in the detection field, can solve the problems of large amount of consumables, poor stability, and high cost, and achieve the effects of improving detection sensitivity, increasing signal, and saving reagent consumption

Inactive Publication Date: 2019-01-25
NINGBO HEIGER ELECTRICAL APPLIANCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for discrete biosensing detection of heavy metals in industrial products. The detection limit of this method has the advantages of universality, high stability, high sensitivity, simple operation, etc., and meets the detection requirements for various heavy metals in industrial products.

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  • Discrete type biological sensing detection method for heavy metals in industrial products
  • Discrete type biological sensing detection method for heavy metals in industrial products
  • Discrete type biological sensing detection method for heavy metals in industrial products

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[0035] In order to enable those skilled in the art to better understand the solution of the present invention, the technical solution of the present invention will be further described below in conjunction with specific examples.

[0036] Below in conjunction with specific embodiment the present invention is described in detail

[0037] The specific example takes Hg as an example, the specific operation method is as follows:

[0038] Preparation of functionalized nanomaterials

[0039] (1) Preparation of up-conversion fluorescent nanomaterials: Weigh YCl 3 ·6H 2 O 0.236g, YbCl 3 ·6H 2 O 0.077g, ErCl 3 ·6H 2 O 0.007g (Ln: 78% Y 3+ , 20%Yb 3+ , 2% Er 3+ ; a total of 1mmol) into a 100mL three-necked flask, add 4mL OA and 15mL 1-ODE, 1500r / min magnetic stirring while gradually warming up to 130 ° C, nitrogen protection to remove water vapor therein, further warming up to 160 ° C, to the mixture Cool to room temperature naturally after forming a homogeneous solution; accu...

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Abstract

The invention discloses a discrete type biological sensing detection method for heavy metals in industrial products. The method comprises the following steps of 1, preparing an up-conversion nano-material, carrying out surface modification, and coupling the gold nano-material to an aptamer; 2, fully mixing and reacting a to-be-detected sample with a proper amount of functionally modified gold nanoparticles, then adding the up-conversion material, and standing for enabling the heavy metals in the sample and the up-conversion material modified with an aptamer complementary chain to competitivelyidentify the aptamer; and 3, measuring a fluorescence value of the up-conversion material by adopting a fluorescence instrument, and by utilizing the characteristic that the fluorescence of the up-conversion material cannot be quenched by the gold nanoparticles combined with the heavy metals, obtaining the content of the heavy metals in the sample. According to the method, the problems of poor specificity, low stability, high material consumption, high manufacturing cost and the like in an existing rapid heavy metal detection technology are solved, and meanwhile, the detection limit of the heavy metals is improved; and the method has the advantages of being universal, high in stability, high in sensitivity, simple to operate and the like, and meets the detection requirements on various heavy metals in the industrial products.

Description

technical field [0001] The invention belongs to the technical field of detection and relates to a detection method of a discrete biosensor for heavy metals in industrial products. Background technique [0002] The source of heavy metals is mainly due to their production during mining, transportation, refining, and processing. In the mining, refining, and use of energy resources such as coal and oil, there will also be the presence and pollution of heavy metals. These heavy metals enter the atmosphere, water, and soil, and then enrich continuously with biological action. Heavy metal pollution is different from pollution from other organic compounds. Heavy metals are enriched and difficult to degrade in the environment. Therefore, heavy metal pollution will cause great harm to human health. [0003] The traditional detection methods of heavy metals mainly include liquid chromatography, gas chromatography, gas-liquid mass spectrometry and so on. Although these methods have ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 丁利许宙康震清江王季诚李明安
Owner NINGBO HEIGER ELECTRICAL APPLIANCES
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