Tissue culture solution of hemsleya amabilis and culture method thereof

Abstract

The invention discloses a tissue culture solution of hemsleya amabilis and a culture method thereof. The tissue culture solution is prepared from a MS medium, 1-2 mg / L of 6-BA, 0.5 mg / L of NAA, 0.5-1.5 mg / L of IBA, 100g / L of banana, 25g / L of white sugar and 1% of activated carbon. The culture method comprises: selecting a MS medium, carrying out rooting induction and seedling strengthening throughphytohormones of NAA and IBA, and simultaneously, feeding a 6-BA composition for induced rooting. The use amount of an inorganic salt and sugar is reduced so that the osmotic potential in the mediumis increased, the free energy of the water is increased and the water potential of the medium is increased and thus water absorption of plants is promoted. The banana can well promote hemsleya amabilis growth. The activated carbon can adsorb many organic and inorganic molecules from the medium to eliminate the bad substances produced during the metabolism of hemsleya amabilis, prevent browning ofexplants and promote rooting, and is conducive to growth.

Description

technical field [0001] The invention relates to the field of snow gall culture, in particular to a tissue culture liquid of snow gall and a breeding method thereof. Background technique [0002] In the rapid proliferation stage of tissue culture of snow gallbladder, the proliferation coefficient is large, and the resulting seedlings are small or of poor quality. It is necessary to strengthen the seedlings. In the rooting culture, the appropriate hormone species and concentration ratio are selected, so that the tissue culture seedlings are still robust while rooting. The existing snow gallbladder tissue culture is in the propagation stage, and the concentration of mitogen is high in the early stage, and the proliferating buds produced are small or of poor quality. If the small buds used for rooting are small or of poor quality, it is not conducive to rooting. Transplanting of seedlings will also have a greater impact on the low survival rate. At present, the method used is t...

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Problems solved by technology

There is no increase in the number of simple seedlings, and the corresponding increase in work links, including the preparation of medium, transfer costs, and the cost of medium, will also increase the risk of pollution and indirectly affect production costs

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