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Growth-promoting bacteria for promoting growth of alfalfa on saline and alkaline land and microbial organic fertilizer thereof

A technology of organic fertilizers and growth-promoting bacteria, applied in the directions of organic fertilizers, microorganism-based methods, microorganisms, etc., can solve problems such as the limitation of improvement effect, improve the ability of colonization and function, improve the growth environment, and improve soil. The effect of the microflora

Active Publication Date: 2019-02-19
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

By applying organic fertilizers, soil physical and chemical properties can be improved to a certain extent, and soil fertility can be improved, but the improvement effect has certain limitations.

Method used

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  • Growth-promoting bacteria for promoting growth of alfalfa on saline and alkaline land and microbial organic fertilizer thereof
  • Growth-promoting bacteria for promoting growth of alfalfa on saline and alkaline land and microbial organic fertilizer thereof
  • Growth-promoting bacteria for promoting growth of alfalfa on saline and alkaline land and microbial organic fertilizer thereof

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Embodiment Construction

[0038] (1) Isolation and screening of growth-promoting strains in saline-alkali soil

[0039] Isolation of growth-promoting strains: the soil used is saline-alkali soil (pH is 8.7, conductivity is 0.54mS cm -1 ), collected from Dongying Comprehensive Experimental Station of Shandong Academy of Agricultural Sciences (118°38'15" north latitude, 37°18'26" east longitude), the dilution coating method was used to screen Trichoderma strains, and a total of 87 Trichoderma strains were obtained All strains were stored in glycerol at -80°C.

[0040] Primary screening and secondary screening of growth-promoting strains: Since the main growth-promoting mechanism of Trichoderma is competition, which is closely related to the growth rate of Trichoderma, the growth rate of Trichoderma was used as the primary screening index, and 7 strains were obtained Representative strains are NAU-T8, NAU-T11, NAU-T14, NAU-25, NAU-T46, NAU-T58, NAU-T78. On this basis, the effect on the growth of alfalfa...

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Abstract

The invention discloses a growth-promoting bacterial strain for the growth of alfalfa on a saline and alkaline land and a microbial organic fertilizer thereof, and belongs to an intensive production technology of animal husbandry. According to the growth-promoting bacterial strain for the growth of the alfalfa on the saline and alkaline land and the microbial organic fertilizer thereof, the growth-promoting bacterial strain for promoting the growth of the alfalfa is obtained through separation, the bacterial strain NAU-T14 is classified and named as Trichoderma harzianum and is preserved in the China Center for Type Culture Collection, and the preservation number of the bacterial strain is CCTCC M 2018520. The growth-promoting biological organic fertilizer prepared by utilizing the bacterial strain fermentation is rich in nutrition, can effectively increase the diversity of soil microorganisms and significantly promotes the growth of the alfalfa.

Description

technical field [0001] The invention relates to a growth-promoting bacterial strain for promoting the growth of alfalfa in saline-alkali land and a microbial organic fertilizer thereof, belonging to the technical field of animal husbandry intensive production. Background technique [0002] Alfalfa is a forage grass widely planted in my country, and has the highest nutritional value among all common forage crops. On saline-alkali land, the growth of alfalfa was obviously inhibited due to the limitation of soil nutrients and structure. At present, China has a large amount of saline-alkali land, which is distributed in Northwest China, North China, the western part of Northeast China and the coastal areas. The soil has high salinity, strong alkalinity, weak fertility, and a very fragile ecological environment. It has become an obstacle to the development of agriculture and animal husbandry in the region. major barriers. By applying organic fertilizers, soil physical and chemi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05G1/00C12R1/885
CPCC12N1/14C05C9/00C12R2001/885C12N1/145C05F3/00C05F11/00C05F11/08
Inventor 张风革许茜茜肖燕王国良李昕芫
Owner NANJING AGRICULTURAL UNIVERSITY
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