Application of fumarate and pharmaceutically acceptable salt thereof in preparation of medicines for ferroptosis related diseases
A kind of technology of fumarate and dimethyl fumarate, applied in the field of chemical medicine
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Embodiment 1
[0048] In vitro inhibition of hepatic ferroptosis assay:
[0049] (1) GSH and GSSG level determination test:
[0050] The monolayer of adherent HepG2 cells was digested with trypsin to form a single cell suspension, and the cells were plated in 96-well plates at 4000 cells / well. After 4-6 hours of cell attachment, add DMSO, 10 μM dimethyl fumarate, 30 μM dimethyl fumarate, 10 μM monomethyl fumarate, and 30 μM monomethyl fumarate to the cell culture plate. Methyl ester was incubated for 20 h, and the GSH / GSSG-GloTM Assay kit (Promega, V6611) was used to detect the total GSH and GSSH of the cells. The specific operation was performed according to the instructions, and the experiment was repeated three times. The result is as figure 1 and figure 2 shown.
[0051] It can be seen from the results in the figure that dimethyl fumarate and monomethyl fumarate can significantly up-regulate the GSH level and GSSH level of HepG2 cells in a concentration-dependent manner, and the up-...
Embodiment 2
[0062] Inhibition test of hepatic ferroptosis in alcoholic fatty liver disease model mice:
[0063] Twenty-four 8-week-old SPF grade C57BL / 6 male mice were randomly divided into 4 groups, namely blank group, model group, model + low-dose dimethyl fumarate (100mg / Kg) group and model + high-dose fumarate group Acetate dimethyl ester (200mg / Kg) group. The mice initially drank the Lieber-DeCarli control diet freely for 5 days to make them gradually adapt to the liquid diet and tube feeding. Subsequently, the model group was allowed to freely drink the liquid diet containing 5% (volume / volume) Lieber-DeCarli alcohol for 10 days, while the control group The group was given an isocaloric control diet. Starting from the 11th day, the model+administration group was given dimethyl fumarate (100 mg / Kg) and dimethyl fumarate (200 mg / Kg) by intragastric administration once a day, respectively, for 10 consecutive days. Afterwards, mice were anesthetized with 5% chloral hydrate intraperito...
Embodiment 3
[0078] Inhibition test of hepatic ferroptosis in rats with liver fibrosis:
[0079] 36 7-week-old 200-250g SPF grade SD rats were randomly divided into 4 groups, namely control group, model group, model+low dose dimethyl fumarate (15mg / Kg) group and model+high dose Dimethyl fumarate (25mg / Kg) group. The model group was established by subcutaneous injection of 40% CCl4 peanut oil solution 0.3ml / 100g, twice a week. From the second week, the mice were given 10% alcohol as the only drink and fed with cornmeal feed mixed with 0.5% cholesterol, and the models were built continuously for 8 weeks. Simultaneously with modeling, the model + drug administration group were given dimethyl fumarate (15 mg / Kg) and dimethyl fumarate (25 mg / Kg) by intragastric administration once a day, respectively, for 8 consecutive weeks. After the mice were anesthetized intraperitoneally with 5% chloral hydrate, the liver tissues were collected, some were fixed in formalin, and some were preserved in liq...
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