A group of lncRNA molecular markers related to pancreatic cancer and its application

A marker, pancreatic cancer technology, applied in the direction of recombinant DNA technology, DNA/RNA fragments, biochemical equipment and methods, etc., to achieve the effect of improving low specificity and low sensitivity, and good labeling effect

Active Publication Date: 2021-11-30
AFFILIATED HOSPITAL OF JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

LncRNA detection in blood has the advantages of convenient sample collection and simple detection methods. At present, there are no reports on the LncRNA characteristically expressed in pancreatic cancer in peripheral blood. The research on the regulatory mechanism of LncRNA markers in pancreatic cancer is still in progress. In its infancy

Method used

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  • A group of lncRNA molecular markers related to pancreatic cancer and its application
  • A group of lncRNA molecular markers related to pancreatic cancer and its application
  • A group of lncRNA molecular markers related to pancreatic cancer and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] RT-PCR experiment of long-chain non-coding RNA in blood, the specific steps are:

[0031] 1. Sample Collection:

[0032] Take 15 cases of blood samples from the normal control group, non-cancer control group and cancer group respectively and place them in EDTA anticoagulant collection tubes for centrifugation at a speed of 1200g for 10 minutes. Pipette the supernatant plasma and place it in a 2ml enzyme-free centrifuge tube; centrifuge again , 12000g, 10min, collect the supernatant plasma in a new 2ml enzyme-free centrifuge tube, discard the sediment at the bottom, and store in a -80°C refrigerator.

[0033] 2. Extraction of RNA:

[0034] Take 5 mL of whole blood and place it in an EDTA anticoagulant collection tube, invert the tube 6-9 times, and centrifuge the tube at -4°C for 10 min at a speed of 1200 g; take out the tube gently, and pipette the supernatant Transfer to a 1.5ml nuclease-free tube; centrifuge again at 12,000g for 10 minutes; pipette the supernatant ...

Embodiment 2

[0055] Preliminary analysis of the evaluation value of LncRNA markers ABHD11-AS1, LINC00176 and SNHG11; the difference between normal and healthy LncRNA markers ABHD11-AS1, LINC00176 and SNHG11 was evaluated by the area under the curve (AUC) of the receiver operating characteristic curve (ROC) Competence in humans and pancreatic cancer. figure 2 It is the value analysis result graph of pancreatic cancer of LncRNA markers ABHD11-AS1, LINC00176 and SNHG11; among them, figure (a) is LncRNA marker ABHD11-AS1, (b) is LncRNA marker LINC00176, (c) is LncRNA marker SNHG11; The ability of LncRNA markers ABHD11-AS1, LINC00176 and SNHG11 to distinguish normal healthy people from pancreatic cancer was evaluated by the area under the curve (AUC) of the ROC curve. Such as figure 2 As shown, the AUC value of ABHD11-AS1 was 0.887 (95% confidence interval (CI): 0.839-0.936, P<0.0001), the AUC value of LINC00176 was 0.707 (95% CI: 0.614-0.800, P<0.0001), and SNHG11 The AUC value was 0.79 (9...

Embodiment 3

[0057] Comparison of the diagnostic value of LncRNA markers ABHD11-AS1, LINC00176 and SNHG11 with conventional tumor indicators

[0058] image 3 It is the analysis chart of pancreatic cancer diagnostic value of ABHD11-AS1 and conventional tumor markers CEA, CA199 and CA125; among them, figure (a) is the diagnosis analysis chart of ABHD11-AS1 and CEA, (b) is the diagnosis of ABHD11-AS1 and CA199 Analysis chart, picture (c) is the diagnostic analysis chart of ABHD11-AS1 and CA125; image 3 As shown, the AUC of ABHD11-AS1 was 0.888 (95% CI: 0.838-0.942, P<0.0001), the AUC of CEA was 0.826 (95% CI: 0.759-0.893, P<0.0001), and the AUC of CA199 was 0.886 (95 %CI: 0.829), the AUC of CA125 was 0.861 (95%CI: 0.785-0.937, P<0.0001), which shows that the diagnostic value of ABHD11-AS1 is higher than the existing tumor markers.

[0059] The combination of lncRNA marker ABHD11-AS1 with tumor markers such as CEA, CA199, and CA125 provides a more effective diagnostic value for the diagnos...

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Abstract

The invention relates to the field of biological detection, in particular to biological molecular detection, and more specifically to a group of LncRNA markers related to pancreatic cancer and applications thereof. The long-chain non-coding RNA molecular markers provided by the present invention include ABHD11-AS1, LINC00176 and SNHG11. Products containing the molecular markers described in the present invention can more accurately and quickly determine whether a subject has suffered from pancreatic cancer or provide information assessment on whether there is a risk of pancreatic cancer, so as to achieve early detection of colorectal cancer, Early treatment; it has an important application prospect in the preparation of products for early assessment of pancreatic cancer or screening of tumor diagnostic drugs for pancreatic cancer.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to biological molecular detection, and more particularly to LncRNA molecular markers related to pancreatic cancer and applications thereof. Background technique [0002] Pancreatic cancer is one of the most common malignant tumors of the digestive tract in the world, and it is also one of the most common malignant tumors in my country. Pancreatic cancer is generally difficult to detect in the early stage, and most patients with pancreatic cancer still have no obvious symptoms when the disease enters the advanced stage. When the symptoms come to the doctor, the timing of surgery is often lost, and due to its tendency to metastasize and resistance to cytotoxic chemotherapy drugs, traditional non-surgical treatments are usually ineffective, and the prognosis is extremely poor. Therefore, improving the early diagnosis level of pancreatic cancer and finding reliable observation indicat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 徐岷龚爱华冯雯凌陈周改刘雅雯蒋小猛徐萍陈宝定黄红梅王慧之
Owner AFFILIATED HOSPITAL OF JIANGSU UNIV
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