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Iris ensata root induced callus culture method

A technology of callus and Yuchan flower, applied in the field of plant tissue culture, can solve the problem of low callus induction rate of Yuchan

Active Publication Date: 2019-03-12
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the callus induction rate of Yuchan flower is not high, and no one has used the mature section of the root of Yuchan flower as explants to induce callus. Transformation system lays the foundation

Method used

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  • Iris ensata root induced callus culture method
  • Iris ensata root induced callus culture method
  • Iris ensata root induced callus culture method

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Embodiment Construction

[0018] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.

[0019] The method for inducing callus culture from the roots of Cicada japonica provided by the present embodiment comprises the following steps:

[0020] Selection and sterilization of seeds: Select mature and cracked seed pod seeds, put them in a conical flask, add an appropriate amount of water and detergent, shake them continuously for 10 minutes to remove impurities, and then rinse them under running water for 1 hour without foaming. After soaking in tap water with an initial temperature of 40 °C for 1 d, the seeds were placed in the sterilized conical flask in a sterile operation table, sealed with sterile gauze, sterilized with 75% alcohol for 1 min, and rinsed with sterile water for 3 times, 4% NaClO was disinfected for 30 min, and washed with sterile water fo...

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Abstract

The invention provides an iris ensata root induced callus culture method. Root segments of maturation zones with fibrous roots of iris ensata roots are used as explants. The iris ensata root induced callus culture method includes disinfecting mature seeds; inoculating the mature seeds in seed germination culture media with 0.5 mg / L of MS+6-BA and the seed germination rates of approximately 100%; inoculating each seedling into rooting culture media when the seedling with 2-3 leaves grows, and allowing the seedlings to grow to obtain aseptic seedlings with developed root systems; cutting root segments of maturation zones with fibrous roots (partially cutting off the fibrous roots and exposing wound) into induction culture media. The lengths of the root segments inoculated into the inductionculture media are 1.0-1.5 cm. The optimal induction culture media are 1.0 mg / L of MS+6-BA, 2 mg / L of 4-D and 0.2 mg / L of NAA, and the induction rate of the induction culture media can reach 100% on the 30th day. The iris ensata root induced callus culture method has the advantage that a solid foundation can be laid for optimizing tissue culture systems and establishing genetic transformation systems.

Description

technical field [0001] The invention relates to a method for inducing callus culture from the roots of Cicada japonica, and relates to the technical field of plant tissue culture. Background technique [0002] Jade cicada (Iris ensata) is a perennial herb of the genus Iris of the family Iridaceae. It has bright and abundant flowers, beautiful plant type, strong cold resistance and high ornamental value. As a garden greening and flower arrangement, it has a very high market potential. It has a wide range of uses in gardens, mainly used in ornamental vegetation under forest, green space planting, grass embellishment, patterned flower beds, etc.; jade cicada can be used as a potted flower in indoor small environment home decoration, and can also be used as a flower arrangement material. [0003] Tissue culture is widely used because it can efficiently propagate plants and can improve plant quality (Shimizu, K. et al.). E.V.Boltenkov and E.V.Zarembo used jade cicada seeds as ex...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王玲刘红艳张捷
Owner NORTHEAST FORESTRY UNIVERSITY
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