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A preparation method and application of earthworm supernatant protein nano-collagen repair compound

A technology of albumin and complexes, applied in drug delivery, pharmaceutical formulations, prostheses, etc., can solve the problems of frequent dressing changes, large consumption, high economic cost, etc., to enhance biocompatibility, promote collagen repair, less toxic effect

Active Publication Date: 2021-05-18
SHAANXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, protein is a biomacromolecule that is very easy to degrade in nature, which is very unfavorable for storage and transportation
In the actual medical process, there are also problems such as frequent dressing changes (large consumption) and high economic costs.

Method used

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  • A preparation method and application of earthworm supernatant protein nano-collagen repair compound
  • A preparation method and application of earthworm supernatant protein nano-collagen repair compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] S1. Preparation of earthworm supernatant protein: wash the fresh and alive Eisenia chinensis with 0.9% NaCl solution for several times, remove the dirt on the body surface, cut the earthworms into pieces, and use a homogenizer to crush the earthworm tissues to obtain earthworm tissue homogenate. Then add sucrose according to the ratio of 10% of the earthworm tissue homogenate quality, stir evenly, centrifuge at 4 ℃ at low speed for 10 minutes after standing still for 30 minutes, get the supernatant and be the earthworm supernatant, and get the earthworm supernatant and adopt the Bradford method ( This method is a classic textbook test method in biochemistry, so this embodiment will not repeat it, mainly for the purpose of measuring the protein concentration, so that in S3, the protein concentration can be converted to the protein amount) After measuring the protein concentration at 20 degrees Celsius, add it to the supernatant 1X anti-protein degrading enzyme to obtain e...

Embodiment 2

[0024] S1. Preparation of earthworm supernatant protein: wash the fresh and alive Eisenia chinensis with 0.9% NaCl solution for several times, remove the dirt on the body surface, cut the earthworms into pieces, and use a homogenizer to crush the earthworm tissues to obtain earthworm tissue homogenate. Then add sucrose according to the ratio of 10% of the mass of earthworm tissue homogenate, stir evenly, centrifuge at 4°C for 10 minutes at a low speed after standing still for 30 minutes, take the supernatant which is the earthworm supernatant, and use the Bradford method to obtain the earthworm supernatant After measuring the protein concentration at 20 degrees Celsius, add 1X anti-protein degrading enzyme to the supernatant to obtain an earthworm supernatant protein solution, and then add 0.02% NaN 3 As a preservative, store for later use;

[0025] S2. Preparation of nano-mesoporous transition metal carrier: Weigh 1 g of a mixture of polyvinylpyrrolidone and sodium dodecylsul...

Embodiment 3

[0028] S1. Preparation of earthworm supernatant: wash the fresh and alive Eisenia worm with 0.9% NaCl solution for several times, remove the dirt on the body surface, cut the earthworm into pieces, and use a homogenizer to crush the earthworm tissue to obtain the earthworm tissue homogenate. Then add sucrose according to the ratio of 10% of the mass of earthworm tissue homogenate, stir evenly, centrifuge at 4°C for 10 minutes at a low speed after standing still for 30 minutes, take the supernatant which is the earthworm supernatant protein, and adopt the Bradford method to obtain the earthworm supernatant protein After measuring the protein concentration at 20 degrees Celsius, add 1X anti-protein degrading enzyme to the supernatant to obtain an earthworm supernatant protein solution, and then add NaN with a concentration of 0.02% 3 As a preservative, store for later use;

[0029]S2. Preparation of nano-mesoporous transition metal carrier: Weigh 1 g of a mixture of polyvinylpyr...

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Abstract

The invention discloses a preparation method and application of an earthworm supernatant protein nano-collagen repair compound, comprising the following steps: preparing earthworm supernatant protein, preparing a nano-mesoporous transition metal carrier, and preparing an earthworm supernatant protein nano-collagen repair compound , the earthworm supernatant nano-collagen repair complex is applied to the collagen repair of animal skin tissue. Compared with the prior art, the present invention inserts effective protein components into the transition metal mesoporous nanomaterials in the form of adsorption force, and uses PLGA to reduce the chance of the active components in contact with the air, while preserving the efficacy of the drug, it also has The biocompatibility is enhanced and the toxicity is reduced; thereby a slow-release, high-efficiency, economical and biocompatible medical pharmaceutical preparation is prepared to achieve the purpose of promoting collagen repair.

Description

technical field [0001] The invention relates to the technical field of animal nano-Chinese medicine preparation, in particular to a preparation method and application of an earthworm supernatant protein nano-collagen repair compound. Background technique [0002] In daily life, various traumatic events occur from time to time. With the development of society and economy, the number of trauma cases caused by traffic accidents, medical trauma and other reasons is also increasing year by year. When trauma occurs, organisms will immediately start their own defect repair mechanism. Wound repair is generally considered to be divided into three distinct phases: the inflammatory phase, the proliferative phase, and the maturation phase. From the inflammatory phase, injured tissue can begin to secrete collagen for collagen deposition. After about three days, wound repair enters the proliferative phase, when the tissue secretes type III and type V collagen. At this time, the collag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/60A61L27/22A61L27/50A61L27/18A61L27/56A61L27/04A61L27/54
CPCA61L27/042A61L27/045A61L27/047A61L27/18A61L27/227A61L27/50A61L27/54A61L27/56A61L27/60A61L2300/252A61L2300/412A61L2300/602A61L2400/12C08L89/00C08L67/04
Inventor 王东谷婷刘韦王瑞辉张红
Owner SHAANXI UNIV OF CHINESE MEDICINE
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