ELISA kit for detecting some animal pathogenic antibody

Abstract

The invention relates to the technical field of animal pathogenic antibody detection and discloses an ELISA kit for detecting some animal pathogenic antibody. The ELISA kit comprises a pre-coated plate, a to-be-detected antibody labeled by a marker enzyme, a coating buffer solution, a washing buffer solution, a terminating solution, a primer buffer solution, a TMB using solution and an ABTS usingsolution. The pre-coated plate is a 96 porous polystyrene board, the marker enzyme is horse radish peroxidase, the coating buffer solution is a carbonate buffer solution, the pH value of which is 9.6,the coating buffer solution is prepared by mixing 1.59 g of Na2CO3, 2.93g of NaHCO3 and 1000 mL distilled water, and the washing buffer solution is a phosphate buffer solution, the pH value of whichis 7.4. The ELISA kit for detecting some animal pathogenic antibody can extract a to-be-detected antibody from feces of a pig without fixing the pig and sampling blood serum from the pig, so that theworking flow is simplified, and the influence on healthy growth of the pig when the blood serum is sampled is avoided effectively.

Description

technical field [0001] The invention relates to the technical field of detection of animal pathogenic antibodies, in particular to an ELISA kit for detecting certain animal pathogenic antibodies. Background technique [0002] Viral infectious diseases have become a serious obstacle to the healthy development of pig breeding in my country, and their outbreak and prevalence have caused great economic losses to pig farmers. At present, swine fever virus, porcine circovirus type 2 and porcine pseudorabies virus are the key viral diseases in the pig industry. In the prevention of pig diseases, real-time detection and evaluation of antibody levels in serum after vaccine immunization and identification of antibody sources are the key links to evaluate the effect of vaccination and protect the health of pigs. Serum antibody detection methods for porcine viral infectious diseases mainly include enzyme-linked immunosorbent assay (ELISA), latex agglutination test, hemagglutination tes...

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies in the prior art, the present invention provides an ELISA kit for detecting certain animal pathogenic antibodies, which can directly extract the required antibody to be tested from pig feces, simplifies the work process, and effectively avoids the need to take serum Sometimes it has the advantages of affecting the healthy growth of pigs. It solves the problem that in indirect ELISA, the pigs must be fixed manually before the serum can be collected. It is troublesome to fix the pigs manually, and taking serum from pigs will also affect the healthy growth of pigs. problems that have a certain impact