ELISA kit for detecting some animal pathogenic antibody

A kit and antibody technology, applied in the field of animal pathogenic antibody detection, can solve the problems of pig fixation trouble and the impact of healthy growth of pigs, and achieve the effect of simplifying the work process and avoiding the impact of healthy growth.

Inactive Publication Date: 2019-03-19
广州动佰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies in the prior art, the present invention provides an ELISA kit for detecting certain animal pathogenic antibodies, which can directly extract the required antibody to be tested from pig feces, simplifies the work process, and effectively avoids the need to take serum Sometimes it has the advantages of affecting the healthy growth of pigs. It solves the problem that in indirect ELISA, the pigs must be fixed manually before the serum can be collected. It is troublesome to fix the pigs manually, and taking serum from pigs will also affect the healthy growth of pigs. problems that have a certain impact

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] ELISA kit for detecting certain animal pathogenic antibodies, including pre-coated plate, antibody to be tested labeled with labeled enzyme, coating buffer, washing buffer, stop solution, substrate buffer, TMB use solution, ABTS use solution , the pre-coated plate is a 96-well polystyrene plate, and the labeled enzyme is horseradish peroxidase.

[0028] The coating buffer is a carbonate buffer with a pH value of 9.6, and the coating buffer consists of 1.59g of Na 2 CO 3 , 2.93g of NaHCO 3 Prepared by mixing with 1000mL of distilled water.

[0029] The washing buffer is a phosphate buffered saline solution with a pH value of 7.4, and the washing buffer is composed of 0.2 g of KH 2 PO 4 , 2.9g of Na 2 HPO 4 、Na 2 HPO 4 12H 2 O, 0.8g of NaCl, 0.2g of KCl, 0.5mL of 0.05% Tween 20 and 1000mL of distilled water were mixed.

[0030] The stop solution is H 2 SO 4 solution, the stop solution was prepared by mixing 178.2mL of distilled water and 21.8mL of 98% concentr...

Embodiment 2

[0041] ELISA kit for detecting certain animal pathogenic antibodies, including pre-coated plate, antibody to be tested labeled with labeled enzyme, coating buffer, washing buffer, stop solution, substrate buffer, TMB use solution, ABTS use solution , the pre-coated plate is a 96-well polystyrene plate, and the labeled enzyme is horseradish peroxidase.

[0042] The coating buffer is a carbonate buffer with a pH value of 9.6, and the coating buffer consists of 1.59g of Na 2 CO 3 , 2.93g of NaHCO 3 Prepared by mixing with 1000mL of distilled water.

[0043] The washing buffer is a phosphate buffered saline solution with a pH value of 7.4, and the washing buffer is composed of 0.2 g of KH 2 PO 4 , 2.9g of Na 2 HPO 4 、Na 2 HPO 4 12H 2 O, 0.8g of NaCl, 0.2g of KCl, 0.5mL of 0.05% Tween 20 and 1000mL of distilled water were mixed.

[0044] The stop solution is H 2 SO 4 solution, the stop solution was prepared by mixing 178.2mL of distilled water and 21.8mL of 98% concentr...

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Abstract

The invention relates to the technical field of animal pathogenic antibody detection and discloses an ELISA kit for detecting some animal pathogenic antibody. The ELISA kit comprises a pre-coated plate, a to-be-detected antibody labeled by a marker enzyme, a coating buffer solution, a washing buffer solution, a terminating solution, a primer buffer solution, a TMB using solution and an ABTS usingsolution. The pre-coated plate is a 96 porous polystyrene board, the marker enzyme is horse radish peroxidase, the coating buffer solution is a carbonate buffer solution, the pH value of which is 9.6,the coating buffer solution is prepared by mixing 1.59 g of Na2CO3, 2.93g of NaHCO3 and 1000 mL distilled water, and the washing buffer solution is a phosphate buffer solution, the pH value of whichis 7.4. The ELISA kit for detecting some animal pathogenic antibody can extract a to-be-detected antibody from feces of a pig without fixing the pig and sampling blood serum from the pig, so that theworking flow is simplified, and the influence on healthy growth of the pig when the blood serum is sampled is avoided effectively.

Description

technical field [0001] The invention relates to the technical field of detection of animal pathogenic antibodies, in particular to an ELISA kit for detecting certain animal pathogenic antibodies. Background technique [0002] Viral infectious diseases have become a serious obstacle to the healthy development of pig breeding in my country, and their outbreak and prevalence have caused great economic losses to pig farmers. At present, swine fever virus, porcine circovirus type 2 and porcine pseudorabies virus are the key viral diseases in the pig industry. In the prevention of pig diseases, real-time detection and evaluation of antibody levels in serum after vaccine immunization and identification of antibody sources are the key links to evaluate the effect of vaccination and protect the health of pigs. Serum antibody detection methods for porcine viral infectious diseases mainly include enzyme-linked immunosorbent assay (ELISA), latex agglutination test, hemagglutination tes...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N1/04
CPCG01N1/04G01N33/558
Inventor 王冠杨忠艳
Owner 广州动佰生物科技有限公司
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