Method for preparing anti-tumor traditional Chinese medicine extract by using high-speed countercurrent chromatography technology

A high-speed countercurrent chromatography, anti-tumor effect technology, used in anti-tumor drugs, drug combinations, pharmaceutical formulations, etc., can solve the problem of few research reports on anti-tumor effects, and achieve significant anti-cancer effects.

Active Publication Date: 2019-03-29
珠海希望基因医药研究院有限公司
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Problems solved by technology

[0003] At present, there are not many research reports on the active ingred

Method used

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  • Method for preparing anti-tumor traditional Chinese medicine extract by using high-speed countercurrent chromatography technology

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Effect test

Embodiment 1

[0026] The preparation of embodiment 1 Chinese medicine extract

[0027] Take the Chinese herbal medicine Dingguicao, heat and reflux extract once with ethanol with a volume fraction of 95%, and the extraction time is 1 hour; concentrate the extract to remove the solvent to obtain the Dingguicao alcohol extract; wherein the Chinese medicinal material Dingguicao and ethanol aqueous solution The dosage ratio is 1g: 15mL;

[0028] (2) Add water to suspend the bucuro alcohol extract, then extract twice with chloroform and ethyl acetate successively, combine the ethyl acetate extraction parts obtained by the two extractions, concentrate and remove the ethyl acetate solvent, and obtain ethyl acetate Ester extract; Wherein the consumption ratio of butyricol extract and water is 1g:20mL; The volume consumption ratio of the volume consumption of chloroform and ethyl acetate and water in each extraction process is 1:1;

[0029] (3) The ethyl acetate extract is separated by high-speed c...

experiment example

[0055] Experimental example: Anti-tumor activity test of traditional Chinese medicine extracts

[0056] Human nasopharyngeal carcinoma cells CNE1 and human lung cancer cells A549 were cultured in culture medium (RPMI 1640 medium), and the cancer cells were cultured in 10×10 3 Add each well to a 96-well plate at 37 °C with 5% CO 2 After culturing for 24 hours in the incubator, the original culture medium was aspirated after attaching to the wall. Then the blank group was added with RPMI 1640 medium containing 10% fetal bovine serum; the experimental group was added with RPMI 1640 medium containing 0.01-100 μg / mL of the drug to be tested respectively; Continue culturing for 4 hours, remove the supernatant, add 100 μL of DMSO, place in the dark for 10 minutes, use a microplate reader (Sunrise Company) to measure the absorbance at 570 nm, and calculate the cell survival according to the absorbance, and set 6 replicates for each treatment hole. Cell viability (%) = ΔOD 药物处理组 / Δ...

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Abstract

The invention relates to the technical field of traditional Chinese medicines, and particularly discloses a method for preparing an anti-tumor traditional Chinese medicine extract by using a high-speed countercurrent chromatography technology. The method comprises the following steps: (1) taking traditional Chinese medicine twinleaf zornia herb, performing extracting on the twinleaf zornia herb with alcohol, and concentrating the obtained extracting solution to remove a solvent to obtain a twinleaf zornia herb alcohol extract; (2) adding water into the twinleaf zornia herb alcohol extract forsuspension, sequentially performing extraction with chloroform and ethyl acetate for 1-3 times respectively, taking the ethyl acetate extraction part, and performing concentration to remove the ethylacetate solvent to obtain an ethyl acetate extract; and (3) separating the ethyl acetate extract by adopting high-speed countercurrent chromatography to obtain the traditional Chinese medicine extract. The traditional Chinese medicine extract prepared by the method has the effects of resisting lung cancer and nasopharyngeal carcinoma, and particularly has a more obvious effect on nasopharyngeal carcinoma.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to a method for preparing a traditional Chinese medicine extract with antitumor effect by using high-speed countercurrent chromatography. Background technique [0002] Dingguicao, the name of Chinese medicinal materials, is the whole herb of the leguminous plant Dingguicao. Harvested in summer, the functions and indications are: heat clearing, detoxification, blood stasis removal, cold, high fever convulsions, diarrhea, jaundice, dysentery, malnutrition in children, sore throat, red eyes, boils and swollen toxins, mastitis. [0003] At present, there are not many research reports on the active ingredients in Dingguicao that have anti-tumor effects in the existing literature. However, there are many chemical components in Dingguicao, which effective parts have anti-tumor effects need to be further researched and developed by researchers; in addition, because the...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61P35/00
CPCA61K36/48A61K2236/331A61K2236/39A61K2236/55A61P35/00
Inventor 曹文强杨懋勋王重庆刘荫贞赵柏松
Owner 珠海希望基因医药研究院有限公司
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