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Antrodia camphorata strain and antrodia camphorata liquid fermentation method

A technology of liquid fermentation and Antrodia camphorata, applied in the field of bioengineering, can solve problems such as weak efficacy, weak market competitiveness, and few types of active products, and achieve the effect of simple technical process

Active Publication Date: 2016-08-03
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the disadvantages of deep liquid fermentation of Antrodia camphorata are also very obvious, the biggest of which is that the types and yields of active products are relatively low. This just makes the efficacy of the Antrodia camphorata liquid fermentation mycelium product weaker, and the market competitiveness is not strong
[0005] Patent documents such as CN1456661A (invention name: large-scale liquid submerged fermentation production process of Antrodia camphorata) and CN101803528A (a novel cultivation method of Antrodia camphorata mycelia) disclose a series of cell cultures for producing Antrodia camphorata by fermentation. method, but the cell culture products disclosed in the above-mentioned documents are all Antrodia camphorata mycelia or polysaccharides, and there is no report on the production method of Antrodia camphorata mycelium characteristic active product androquinol compounds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Slant medium: malt juice agar medium.

[0023] Liquid seed medium (g / L): glucose 20, tryptone 4, corn steep liquor 2.

[0024] Liquid fermentation medium (g / L): glucose 20, tryptone 2, corn steep liquor powder 2, K 2 HPO 4 0.1, MgSO 4 0.1. All media used were sterilized at 115°C for 20 min.

[0025] The strains of Antrodia antrodia were first cultured on the wort slope at 28°C for 12 days, and then inserted into the liquid seed medium for culture. After the liquid seeds were cultured at 30°C for 2 days, they were inserted into the liquid fermentation medium.

[0026] The culture conditions for liquid fermentation are: culture temperature 28°C, shaker speed 100r / min, pH 5.0, after 1 day of culture, 5 mmol / L of hydrogen peroxide was added, and then the culture was continued for 5 days. Using this method to stress Antrodia camphorata to produce characteristic active products, the content of Antroquinol is 2 mg / g thallus, and the content of Antroquinol B is 5 mg / g tha...

Embodiment 2

[0028] Slant medium: malt juice agar medium.

[0029] Liquid seed medium (g / L): glucose 20, tryptone 4, corn steep liquor 2.

[0030] Liquid fermentation medium (g / L): glucose 40, tryptone 5, corn steep liquor powder 2, K 2 HPO 4 0.3, MgSO 4 0.3. All media used were sterilized at 115°C for 20 min.

[0031] The strains of Antrodia antrodia were first cultured on the wort slope at 28°C for 12 days, and then inserted into the liquid seed medium for culture. After the liquid seeds were cultured at 30°C for 2 days, they were inserted into the liquid fermentation medium.

[0032] The culture conditions for liquid fermentation are: culture temperature 28°C, shaking table speed 100r / min, pH 5.0, after 3 days of culture, 20mmol / L of hydrogen peroxide was added, and then the culture was continued for 10 days. Using this method to stress Antrodia camphorata to produce characteristic active products, the content of Antroquinol is 10 mg / g thallus, and the content of Antroquinol B is ...

Embodiment 3

[0034] Slant medium: malt juice agar medium.

[0035] Liquid seed medium (g / L): glucose 20, tryptone 4, corn steep liquor 2.

[0036] Liquid fermentation medium (g / L): sucrose 40, soybean powder 5, corn steep liquor powder 2, K 2 HPO 4 0.3, MgSO 4 0.3. All media used were sterilized at 115°C for 20 min.

[0037]The strains of Antrodia antrodia were first cultured on the wort slope at 28°C for 12 days, and then inserted into the liquid seed medium for culture. After the liquid seeds were cultured at 30°C for 2 days, they were inserted into the liquid fermentation medium.

[0038] The culture conditions of liquid fermentation are: culture temperature 28°C, shaker speed 100r / min, pH 5.0, after 6 days of culture, 35mmol / L of hydrogen peroxide is added, and then the culture is continued for 15 days. Using this method to stress Antrodia camphorata to produce characteristic active products, the content of Antroquinol is 13 mg / g thallus, and the content of Antroquinol B is 44 mg...

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Abstract

An antrodia camphorata liquid fermentation method comprises the following steps: A, using antrodia camphorata strain S-29 as a raw material and firstly culturing on a wort slant medium to obtain an antrodia camphorata slant strain; B, inoculating the antrodia camphorata slant strain into a liquid seed medium for cultivation so as to obtain a liquid seed; and C, inoculating the liquid seed into a liquid fermentation medium for culture, adding hydrogen peroxide during the cultivation process and oxidizing stress-induced mycelium through the hydrogen peroxide to synthesize an antroquinonol compound. The method of the invention is simple and efficient and has important industrial application value. Due to addition of hydrogen peroxide for inducing antrodia camphorata liquid fermentation to generate the antroquinonol and its derivative, efficacy gap between the antrodia camphorata liquid mycelium product and other forms of culture products is greatly narrowed. The antroquinonol and its derivative produced by the method can be applied in the field of medicine and health food.

Description

technical field [0001] The invention relates to bioengineering, in particular to a strain of Antrodia camphorata and a method for liquid fermentation of Antrodia camphorata. technical background [0002] Higher medicinal fungus Antrodia camphorata (Antrodiacamphorata), also known as camphor mushroom, belongs to Basidiomycetes, perennial basidiomycetes, and only grows on the inner wall of decayed trunks of camphor trees in Taiwan. In the folk, Antrodia cintrodia has long been used as a traditional prescription for hangover and liver protection, for the treatment of liver disease, food and drug-induced poisoning symptoms, as well as diarrhea, abdominal pain, high blood pressure, itchy skin and tumor diseases, etc., also known as It is the king of Ganoderma lucidum and the ruby ​​in the forest. In recent years, many scholars have shown that Antrodia Antrodia has various active functions such as anti-tumor, liver protection, anti-inflammation, anti-oxidation, and anti-fatigue, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P7/26C12R1/645
Inventor 夏永军艾连中许赣荣王光强杨昳津
Owner UNIV OF SHANGHAI FOR SCI & TECH
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