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The new application of Rendan in the treatment of ras proto-oncogene overexpression tumors

A proto-oncogene and overexpression technology, which is applied in the direction of antineoplastic drugs, plant raw materials, active ingredients of hydroxyl compounds, etc., can solve the problems of strong side effects, drug resistance, and urgent research, and achieve the effect of application safety

Active Publication Date: 2021-12-10
SHANGHAI ZHONGHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The combination of traditional chemotherapy, surgery, and radiotherapy has successfully improved the treatment rate of various malignant tumors, but due to strong side effects and drug resistance, it is urgent to find new treatments for tumors (Zhang Xiuyun, 2012)

Method used

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  • The new application of Rendan in the treatment of ras proto-oncogene overexpression tumors
  • The new application of Rendan in the treatment of ras proto-oncogene overexpression tumors
  • The new application of Rendan in the treatment of ras proto-oncogene overexpression tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Preparation of Extract

[0027] The invention is not particularly limited to the source of the dragon people, and can be obtained from the market. In an embodiment of the invention, the dragon Tiger Dan purchases from Shanghai Zhonghua Pharmaceutical Co., Ltd., [Production Bottom] 160507.

[0028] In addition to the above-mentioned clear use of the name of the product, the "person" of the present invention specifically refers to the Chinese medicine components.

[0029] People's prescriptions of human medicine pharmaceutical units: 40 sheets, 30 bib pieces, 25 lanquil, 15 octagon, 40 cinnamon, 15 white peppers, 25 dried ginger, 25 Tea 200 copies, 3,64.1 licorice.

[0030] Since the present invention employs a screening tool for screening tools, the Ras / MAPK signaling pathway is performed to excessively activate the anti-tumor drug candidate, and therefore, the human medicine is admitted to a human tanol lever and the human volatile extract. Anti-tumor drug scree...

Embodiment 2

[0033] EXAMPOS

[0034]ras excessive activation may cause the nematode Caenorhabditis elegans vulval generate a plurality, in humans are caused by malignant cell proliferation, the test drug can be suppressed if the test nematode plurality vulva phenotype, prompting them to develop into wild-type, then the drug It acts on the Ras pathway, down excessive activation of this pathway, have antitumor activity. MT2124 is let-60 / ras overactivation of Caenorhabditis elegans, nematode strain which is a multi-vulva phenotype equivalent to the human suffering from refractory cancer ras oncogene mutations. Using the test drug-treated nematode MT2124, if the test drug can be suppressed phenotype plurality vulva nematode test, prompting them to develop into wild-type, then the effect of the drug on the Ras pathway, down excessive activation of this pathway, have antitumor activity. Wild type C. elegans higher proportion of the population of the nematode test, the better the effect of the test...

Embodiment 3

[0060] Example Three Dan effect on C. elegans MT8189 (lin-15 mutant) embodiment

[0061] lin-15 is located upstream of ras, the ras gene is suppressed, the lin-15 deletion mutations, ras by inhibiting action of the disappearance of the nematode exhibits multi vulva phenotype. MT8189 (lin-15) contains the C. elegans wild-type let60 / ras copy can be made when Dan Caenorhabditis MT2124 (let60) significantly increased the proportion of wild-type, but can not be suppressed MT8189 (lin-15) nematodes when multiple vulva phenotype described Dan only let-60 ras mutations in tumor cells leads to activation caused by excessive work, while the wild-type ras lin-15 deletion mutations in tumor cells does not work due to excessive activation. Thus, Dante certifier extracts act only on the let-60 ras mutations in tumor cells caused by excessive activation, does not act on normal cells.

[0062] Experimental Materials: Caenorhabditis elegans MT8189, multi vulva phenotype, genotype: lin-15 (n765ts...

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Abstract

The invention discloses a new application of rendan in the preparation of medicaments for treating tumors with overexpression of ras proto-oncogene, in particular a new application of rendan in treating liver cancer, pancreatic cancer, lung cancer and colon cancer. Rendan is a Chinese patent medicine for treating heat stroke and dizziness. The experimental results of the present invention show that Rendan can significantly inhibit the Caenorhabditis elegans tumor-like polyvulva phenotype caused by the overexpression of the ras proto-oncogene caused by the mutation of let-60, and reverse it into a polyvulva with Wild-type phenotype of true vulva. It shows that Rendan has the potential to treat tumors with overexpression of ras gene, and can be used to prepare drugs for treating tumors with overexpression of ras proto-oncogene.

Description

Technical field [0001] The present invention relates to a new use of human benchmakers over expression of the RAS raw carcinoma, belonging to the field of anti-tumor drugs. Background technique [0002] At present, the malignant tumor has become a human number one killer, which seriously endangers the health of humanity, the information of the World Health Organization shows that the onset of tumors around the world is 10 million, and death is about 7 million deaths. [0003] About 30% of human tumors are caused by excessive activation of ras proteins after RAS rascoomic gene mutation. In a 30% human tumor, there is a mutation of the Ras gene and an excessive activation of Ras protein, of which about 90% of pancreatic cancer, 50% of colon cancer and 20% acute leukemia have Ras gene mutation (Hara and Han, 1995) KRAS mutation in 40% hereditary non-polymetry colorectal carcinoma, 49% conventional filter thyroid cancer has Ras mutation, 48% HURTHLE cell thyroid tumors have RAS mutat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/9068A61P35/00A61K31/045
CPCA61K36/9064A61K36/9068A61P35/00A61K31/045A61K36/285A61K36/48A61K36/484A61K36/54A61K36/57A61K36/61A61K36/67A61K2300/00
Inventor 金家骅李红玉丁礼琴支德娟汪弼晔李洋曹无介刘婷朱晓萍王宇
Owner SHANGHAI ZHONGHUA PHARMA