Evaluation method of hemolysis of mesenchymal stem cell preparation and application
A technology of mesenchymal stem cells and evaluation method, which is applied in the field of evaluation of hemolysis of mesenchymal stem cell preparations, can solve problems such as lack of evaluation index technical means, and achieve the effect of avoiding inaccurate evaluation results
Inactive Publication Date: 2019-04-05
TIANJIN CHANGHE BIOLOGICAL TECH
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Benefits of technology
In this patented technology, there are two ways that researchers use different types of samples from bone marrow to evaluate how well they prepare certain substances called mesenchyma stroma (MSC) before administering them into humans. By comparing these sample sizes together, it becomes possible to determine if any changes made during treatment affect the quality of MSC' s ability to fight disease. Additionally, through various methods such as measuring their levels over time, we have found correlative relationships between both quantities and treatments based upon those values. These techniques help us better identify potential side effectors associated with each type of medicine being developed. Overall, our technical result suggests an improved way to study biological properties of MCS preparations while reducing errors introduced when analyzed manually.
Problems solved by technology
This patented method proposes how to prepare high amounts (up to 10 million) of human adult sternum derived stroma cells (MSCs), which has been found useful in research applications such as understanding blood vessel growth and developing new therapies targeting these properties. Additionally, it highlights the importance of maintaining authenticity during storage and use procedures without compromising any harmful side effect(s).
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Embodiment 1
[0072] 1. Experimental design
[0073] 1.1. Concentration of the test product and reason for selection
[0074] Umbilical cord mesenchymal stem cells (MSCs) have multi-lineage differentiation function and can differentiate into various cells. This research intends to use MSCs for clinical treatment of various diseases. The clinical intravenous infusion dose is 1×10 6 cells / kg, clinical administration concentration 6×10 5 cells / ml. The hemolysis test of intravenous administration preparation adopts clinical concentration to test, and the detection concentration of this embodiment is 6 * 10 5 cells / ml.
[0075] Because the test product in this test is a cell suspension with poor transparency, it is necessary to set up a background control tube for the test product to deduct the influence of the background color of the test product on the experimental results.
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The invention provides an evaluation method of hemolysis of a mesenchymal stem cell preparation and application and relates to the technical field of biology. According to the evaluation method of thehemolysis of the mesenchymal stem cell preparation, provided by the invention, mesenchymal stem cell preparations of a variety of volume percents are separately mixed with erythrocyte suspensions, sothat the risk that the result is inaccurate due to an improper concentration due to the fact that a single-dosage concentration is mixed with an erythrocyte suspension is lowered, and meanwhile, therelationship between the hemolysis and dosage of each mesenchymal stem cell preparation can be obtained. The invention provides a variety of reasonable volume percent volumes, and thus, the problem that the evaluation result is inaccurate due to unreasonable dosage is avoided. The evaluation method can provide safety data for mesenchymal stem cell preparations and provide reference for clinical safe medication.
Description
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